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验证数据展示

  • WB analysis using 85069-4-RR

    WB analysis using 85069-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85069-4-RR (Syntaxin 12 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 85069-4-RR

    IF Staining of A431 using 85069-4-RR

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using Syntaxin 12 antibody (85069-4-RR, Clone: 242546H2 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 85069-4-RR

    Affinity and Kinetic Characterization of 85069-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85069-4-RR against Human Syntaxin 12 were performed. The affinity constant is 1.05 nM.

Syntaxin 12 Recombinant antibody

Syntaxin 12 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 85069-4-RR

产品说明书

CloneNo. 242546H2

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

Syntaxin-12, STX12, 242546H2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inLNCaP cells, HepG2 cells, HEK-293 cells, Jurkat cells, human placenta tissue
Positive IF/ICC detected inA431 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85069-4-RR targets Syntaxin 12 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 Syntaxin 12 fusion protein Ag5532 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 syntaxin 12
别名 Syntaxin-12, STX12, 242546H2
计算分子量 32 kDa
观测分子量39 kDa
GenBank蛋白编号BC046999
基因名称 Syntaxin 12
Gene ID (NCBI) 23673
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ86Y82
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Syntaxin 12 (STX12), also known as STX13 or STX14, is a 276 amino acid single-pass type IV membrane protein that belongs to the syntaxin family. It is a broadly and differentially expressed SNARE protein, highly enriched in brain tissue. STX12 is localized to early and recycling endosomes and is involved in the recycling of plasma membrane proteins. It also has a role in the trafficking of MMP during the degradation of ECM substrates and subsequent cellular invasion. (PMID: 9507000; 9553086; 19910495) This antibody recognizes a major 39 kDa band of endogenous STX12. In some cases, two additional bands of 33 kDa and 66 kDa could also be detected, which most likely represent a degradation product and a SNARE complex respectively (PMID: 9817754).

实验方案

Product Specific Protocols
WB protocol for Syntaxin 12 antibody 85069-4-RRDownload protocol
IF protocol for Syntaxin 12 antibody 85069-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols