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验证数据展示

  • WB analysis using 85065-3-RR (same clone as 85065-3-PBS)

    WB analysis using 85065-3-RR (same clone as 85065-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85065-3-RR (DDX46 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85065-3-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP01807-1

    Sandwich ELISA standard curve of MP01807-1

    Sandwich ELISA standard curve of MP01807-1, Human DDX46 Recombinant Matched Antibody Pair - PBS only. 85065-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag10342. 85065-1-PBS was HRP conjugated as the detection antibody. Range: 0.391-25 ng/mL.

  • Sample test of MP01807-1

    Sample test of MP01807-1

    The mean DDX46 concentration was determined to be 109.98 ng/mL in HEK-293 cell extract based on a 2.70 mg/mL extract load and 35.02 ng/mL in HeLa cell extract based on a 1.50 mg/mL extract load.

  • IHC staining of Human Hepatocellular cancer using 85065-3-RR (same clone as 85065-3-PBS)

    IHC staining of Human Hepatocellular cancer using 85065-3-RR (same clone as 85065-3-PBS)

    Immunohistochemical analysis of paraffin-embedded Human Hepatocellular cancer slide using 85065-3-RR (DDX46 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85065-3-PBS in a different storage buffer formulation.

  • IF Staining of U-251 using 85065-3-RR (same clone as 85065-3-PBS)

    IF Staining of U-251 using 85065-3-RR (same clone as 85065-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using DDX46 antibody (85065-3-RR, Clone: 242715E10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85065-3-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01807-1

    Cytometric bead array standard curve of MP01807-1

    Cytometric bead array standard curve of MP01807-1, DDX46 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85065-3-PBS. Detection antibody: 85065-1-PBS. Standard: Ag10342. Range: 0.781-100 ng/mL.

  • Affinity and Kinetic Characterization of 85065-3-RR

    Affinity and Kinetic Characterization of 85065-3-RR

    Biolayer interferometry (BLl) kinetic assays of 85065-3-RR against Human DDX46 were performed. The affinity constant is 51.9 pM.

DDX46 Recombinant monoclonal antibody, PBS Only (Capture)

DDX46 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 85065-3-PBS

产品说明书

CloneNo. 242715E10

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

别名

242715E10, DEAD box protein 46, EC:3.6.4.13, KIAA0801, Probable ATP-dependent RNA helicase DDX46

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

85065-3-PBS targets DDX46 as part of a matched antibody pair:

MP01807-1: 85065-3-PBS capture and 85065-1-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag10342

Product name: Recombinant human DDX46 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 682-1031 aa of BC012304

Sequence: GLDVKHLILVVNYSCPNHYEDYVHRAGRTGRAGNKGYAYTFITEDQARYAGDIIKALELSGTAVPPDLEKLWSDFKDQQKAEGKIIKKSSGFSGKGFKFDETEQALANERKKLQKAALGLQDSDDEDAAVDIDEQIESMFNSKKRVKDMAAPGTSSVPAPTAGNAEKLEIAKRLALRINAQKNLGIESQDVMQQATNAILRGGTILAPTVSAKTIAEQLAEKINAKLNYVPLEKQEEERQDGGQNESFKRYEEELEINDFPQTARWKVTSKEALQRISEYSEAAITIRGTYFPPGKEPKEGERKIYLAIESANELAVQKAKAEITRLIKEELIRLQNSYQPTNKGRYKVL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 DEAD (Asp-Glu-Ala-Asp) box polypeptide 46
别名 242715E10, DEAD box protein 46, EC:3.6.4.13, KIAA0801, Probable ATP-dependent RNA helicase DDX46
计算分子量 1031 aa, 117 kDa
观测分子量140 kDa
GenBank蛋白编号BC012304
基因名称 DDX46
Gene ID (NCBI) 9879
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ7L014
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DDX46, also named as KIAA0801, is a 1031 amino acid protein, which belongs to the DEAD box helicase family. DDX46 has been shown to be required at the early step of pre-spliceosome assembly. It uses the energy released by the hydrolysis of ATP to rearrange local RNA-RNA or protein-RNA interactions. The nuclear DDX46 acted as a negative regulator of the antiviral innate response by recruiting the m6A'eraser' ALKBH5 to induce retention of antiviral innate mRNA in the nucleus.

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