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验证数据展示

  • WB analysis using 84919-6-RR

    WB analysis using 84919-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84919-6-RR (CSF1R/CD115 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse spleen using 84919-6-RR

    IHC staining of mouse spleen using 84919-6-RR

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84919-6-RR (M-CSF R/CD115 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse spleen using 84919-6-RR

    IHC staining of mouse spleen using 84919-6-RR

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84919-6-RR (M-CSF R/CD115 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 84919-6-RR

    Affinity and Kinetic Characterization of 84919-6-RR

    Biolayer interferometry (BLl) kinetic assays of 84919-6-RR against Mouse CSF1R/CD115 were performed. The affinity constant is below 1 pM.

CSF1R/CD115 Recombinant monoclonal antibody

CSF1R/CD115 Uni-rAb® Recombinant Antibody for WB, IHC, ELISA
Cat No. 84919-6-RR

产品说明书

CloneNo. 242213G11

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

WB, IHC, ELISA

别名

Csf1r, CD115, CSF 1R, CSF-1 receptor, Csfmr

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inJ774A.1 cells, RAW 264.7 cells, mouse spleen tissue
Positive IHC detected inmouse spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84919-6-RR targets CSF1R/CD115 in WB, IHC, ELISA applications and shows reactivity with mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 mouse
免疫原

CatNo: Eg1816

Product name: Recombinant Mouse CSF1R protein (rFc Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-rFc

Tag: C-rFc

Domain: 20-511 aa of NM_001037859.2

Sequence: APVIEPSGPELVVEPGETVTLRCVSNGSVEWDGPISPYWTLDPESPGSTLTTRNATFKNTGTYRCTELEDPMAGSTTIHLYVKDPAHSWNLLAQEVTVVEGQEAVLPCLITDPALKDSVSLMREGGRQVLRKTVYFFSPWRGFIIRKAKVLDSNTYVCKTMVNGRESTSTGIWLKVNRVHPEPPQIKLEPSKLVRIRGEAAQIVCSATNAEVGFNVILKRGDTKLEIPLNSDFQDNYYKKVRALSLNAVDFQDAGIYSCVASNDVGTRTATMNFQVVESAYLNLTSEQSLLQEVSVGDSLILTVHADAYPSIQHYNWTYLGPFFEDQRKLEFITQRAIYRYTFKLFLNRVKASEAGQYFLMAQNKAGWNNLTFELTLRYPPEVSVTWMPVNGSDVLFCDVSGYPQPSVTWMECRGHTDRCDEAQALQVWNDTHPEVLSQKPFDKVIIQSQLPIGTLKHNMTYFCKTHNSVGNSSQYFRAVSLGQSKQLPDES

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 colony stimulating factor 1 receptor
别名 Csf1r, CD115, CSF 1R, CSF-1 receptor, Csfmr
计算分子量109 kDa
观测分子量110-170 kDa
GenBank蛋白编号NM_001037859.2
基因名称 CSF1R/CD115
Gene ID (NCBI) 12978
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP09581
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CSF1R (colony-stimulating factor 1 receptor) belongs to the type III protein tyrosine kinase receptor family. CSF1R-mediated signaling is crucial for the differentiation and survival of the mononuclear phagocyte system and macrophages in particular (PMID: 28716061).

实验方案

Product Specific Protocols
IHC protocol for CSF1R/CD115 antibody 84919-6-RRDownload protocol
WB protocol for CSF1R/CD115 antibody 84919-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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