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验证数据展示

  • WB analysis using 84916-6-RR

    WB analysis using 84916-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84916-6-RR (FEN1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 84916-6-RR

    IF Staining of HeLa using 84916-6-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using FEN1 antibody (84916-6-RR, Clone: 242330E12 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 84916-6-RR

    Affinity and Kinetic Characterization of 84916-6-RR

    Biolayer interferometry (BLl) kinetic assays of 84916-6-RR against Human FEN1 were performed. The affinity constant is 10.3 nM.

FEN1 Recombinant antibody

FEN1 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 84916-6-RR

产品说明书

CloneNo. 242330E12

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, ELISA

别名

hFEN 1, Flap structure-specific endonuclease 1, FEN-1, FEN 1, EC:3.1.-.-

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA431 cells, Jurkat cells. HeLa cells, Y79 cells, NIH/3T3 cells
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy FEN1 Ready-To-Use IHC Kit. FEN1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84916-6-RR targets FEN1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 FEN1 fusion protein Ag6552 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 flap structure-specific endonuclease 1
别名 hFEN 1, Flap structure-specific endonuclease 1, FEN-1, FEN 1, EC:3.1.-.-
计算分子量 43 kDa
观测分子量48 kDa
GenBank蛋白编号BC000323
基因名称 FEN1
Gene ID (NCBI) 2237
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP39748
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FEN1(Flap endonuclease-1) is the prototypical member of the 5'-nuclease superfamily, whose activities span a range of cellular pathways involved in DNA replication and genome maintenance (PMID: 22118811, 21496641, 20929870). FEN1 is a structure-selective metallonuclease essential for Okazaki fragment maturation through efficient removal of 5' flaps resulting from strand displacement during lagging-strand synthesis (PMID: 8144677, 9081985). FEN1 is overexpressed in multiple cancer types, and has been suggested both as a biomarker relating to prognosis and disease progression and as a potential therapeutic target (PMID: 19010819, 16879693, 19596913, 27526030).

实验方案

Product Specific Protocols
WB protocol for FEN1 antibody 84916-6-RRDownload protocol
IF protocol for FEN1 antibody 84916-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols