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验证数据展示

  • IHC staining of human colon cancer using 84914-5-RR

    IHC staining of human colon cancer using 84914-5-RR

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84914-5-RR (TCOF1 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TCOF1 antibody included
  • IF Staining of MCF-7 using 84914-5-RR

    IF Staining of MCF-7 using 84914-5-RR

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using TCOF1 antibody (84914-5-RR, Clone: 242201F12 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 84914-5-RR

    Affinity and Kinetic Characterization of 84914-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84914-5-RR against Human TCOF1 were performed. The affinity constant is 8.67 nM.

TCOF1 Recombinant monoclonal antibody

TCOF1 Uni-rAb® Recombinant Antibody for IHC, IF/ICC, ELISA
Cat No. 84914-5-RR

产品说明书

CloneNo. 242201F12

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, IF/ICC, ELISA

别名

242201F12, MFD1, treacle

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy TCOF1 Ready-To-Use IHC Kit. TCOF1 primary antibody included.

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84914-5-RR targets TCOF1 in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag1447

Product name: Recombinant human TCOF1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-350 aa of BC014559

Sequence: MAEARKRRELLPLIYHHLLRAGYVRAAREVKEQSGQKCFLAQPVTLLDIYTHWQQTSELGRKRKAEEDAALQAKKTRVSDPISTSESSEEEEEAEAETAKATPRLASTNSSVLGADLPSSMKEKAKAETEKAGKTGNSMPHPATGKTVANLLSGKSPRKSAEPSANTTLVSETEEEGSVPAFGAAAKPGMVSAGQADSSSEDTSSSSDETDVEGKPSVKPAQVKASSVSTKESPARKAAPAPGKVGDVTPQVKGGALPPAKRAKKPEEESESSEEGSESEEEAPAGTRSQVKASEKILQVRAASAPAKGTPGKGATPAPPGKAGAVASQTKAGKPEEDSESSSEESSDSE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 Treacher Collins-Franceschetti syndrome 1
别名 242201F12, MFD1, treacle
计算分子量 97 kDa, 144 kDa, 152 kDa
GenBank蛋白编号BC014559
基因名称 TCOF1
Gene ID (NCBI) 6949
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ13428
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TCOF1 also known as treacle, is a nucleolar protein with a LIS1 homology domain. TCOF1 is involved in ribosomal DNA gene transcription by interacting with upstream binding factor (UBF). Mutations in TCOF1 have been associated with Treacher Collins syndrome, a disorder which includes abnormal craniofacial development(PMID: 31218872)

实验方案

Product Specific Protocols
IF protocol for TCOF1 antibody 84914-5-RRDownload protocol
IHC protocol for TCOF1 antibody 84914-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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