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验证数据展示

  • WB analysis using 84888-4-RR

    WB analysis using 84888-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84888-4-RR (PLSCR1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84888-4-RR

    Affinity and Kinetic Characterization of 84888-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84888-4-RR against Human PLSCR1 were performed. The affinity constant is 0.377 nM.

PLSCR1 Recombinant antibody

PLSCR1 Recombinant Antibody for WB, ELISA
Cat No. 84888-4-RR

产品说明书

CloneNo. 242372B2

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

phospholipid scramblase 1, MMTRA1B, Mg(2+)-dependent nuclease, EC:3.1.-.-, Ca(2+)-dependent phospholipid scramblase 1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells, HEK-293 cells, A549 cells, MCF-7 cells, Jurkat cells, human placenta tissue
Planning an IHC experiment? We recommend our IHCeasy PLSCR1 Ready-To-Use IHC Kit. PLSCR1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84888-4-RR targets PLSCR1 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 PLSCR1 fusion protein Ag2172 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 phospholipid scramblase 1
别名 phospholipid scramblase 1, MMTRA1B, Mg(2+)-dependent nuclease, EC:3.1.-.-, Ca(2+)-dependent phospholipid scramblase 1
计算分子量 318 aa, 35 kDa
观测分子量34 kDa
GenBank蛋白编号BC021100
基因名称 PLSCR1
Gene ID (NCBI) 5359
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDO15162
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PLSCR1 (Phospholipid scramblase 1) is a member of the family of membrane proteins that mediate the transbilayer movement of phospholipids ("scrambling") in a Ca2+ dependent manner, which was identified as an a type II transmembrane protein involved in the calcium-dependent, nonspecific, rapid redistribution of phospholipids. A cysteine-rich palmitoylation motif [184CCCPCC189] of PLSCR1, a thioester linkage to the sulfhydryl groups of cysteines, controls the transportation of PLSCR1 to the cell membrane or nucleus (PMID: 32292520). PLSCR1 is strongly expressed in response to interferon (IFN) treatment and viral infection (PMID: 35138119, 23487022).

实验方案

Product Specific Protocols
WB protocol for PLSCR1 antibody 84888-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols