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验证数据展示

  • WB analysis using 84871-4-RR

    WB analysis using 84871-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84871-4-RR (ALDH7A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 84871-4-RR

    IHC staining of human ovary cancer using 84871-4-RR

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 84871-4-RR (ALDH7A1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human ovary cancer using 84871-4-RR

    IHC staining of human ovary cancer using 84871-4-RR

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 84871-4-RR (ALDH7A1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 84871-4-RR

    Affinity and Kinetic Characterization of 84871-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84871-4-RR against Human ALDH7A1 were performed. The affinity constant is 0.253 nM.

ALDH7A1 Recombinant antibody

ALDH7A1 Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA
Cat No. 84871-4-RR

产品说明书

CloneNo. 242363B11

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

242363B11, ALDH7A 1, Alpha-AASA dehydrogenase, Alpha-aminoadipic semialdehyde dehydrogenase, Antiquitin-1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, HEK-293T cells, A549 cells, A431 cells, mouse liver tissue, rat liver tissue
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84871-4-RR targets ALDH7A1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 ALDH7A1 fusion protein Ag0383 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 aldehyde dehydrogenase 7 family, member A1
别名 242363B11, ALDH7A 1, Alpha-AASA dehydrogenase, Alpha-aminoadipic semialdehyde dehydrogenase, Antiquitin-1
计算分子量 55 kDa
观测分子量 55 kDa
GenBank蛋白编号BC002515
基因名称 ALDH7A1
Gene ID (NCBI) 501
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP49419
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ALDH7A1(Alpha-aminoadipic semialdehyde dehydrogenase) is also named as ATQ1 and belongs to the aldehyde dehydrogenase family. It plays a major role in the detoxification of aldehydes generated by alcohol metabolism and lipid peroxidation.

实验方案

Product Specific Protocols
WB protocol for ALDH7A1 antibody 84871-4-RRDownload protocol
IHC protocol for ALDH7A1 antibody 84871-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols