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验证数据展示

  • WB analysis using 84859-3-RR (same clone as 84859-3-PBS)

    WB analysis using 84859-3-RR (same clone as 84859-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84859-3-RR (YARS antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84859-3-PBS in a different storage buffer formulation.

  • IHC staining of human ovary cancer using 84859-3-RR (same clone as 84859-3-PBS)

    IHC staining of human ovary cancer using 84859-3-RR (same clone as 84859-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 84859-3-RR (YARS antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84859-3-PBS in a different storage buffer formulation.

  • IF Staining of U2OS using 84859-3-RR (same clone as 84859-3-PBS)

    IF Staining of U2OS using 84859-3-RR (same clone as 84859-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using YARS antibody (84859-3-RR, Clone: 242400E9 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84859-3-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01634-2

    Cytometric bead array standard curve of MP01634-2

    Cytometric bead array standard curve of MP01634-2, YARS Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84859-3-PBS. Detection antibody: 84859-2-PBS. Standard: Ag35148. Range: 0.156-20 ng/mL.

  • Affinity and Kinetic Characterization of 84859-3-RR

    Affinity and Kinetic Characterization of 84859-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84859-3-RR against Human YARS were performed. The affinity constant is 0.414 nM.

YARS Recombinant monoclonal antibody, PBS Only (Capture)

YARS Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 84859-3-PBS

产品说明书

CloneNo. 242400E9

宿主/亚型

Rabbit / IgG

种属反应性

human, pig

应用

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

别名

YARS1, 242400E9, CMTDIC, TYRRS, YRS

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

84859-3-PBS targets YARS as part of a matched antibody pair:

MP01634-2: 84859-3-PBS capture and 84859-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, pig
免疫原

CatNo: Ag35148

Product name: Recombinant human YARS protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 400-528 aa of BC016689

Sequence: RTVVSGLVQFVPKEELQDRLVVVLCNLKPQKMRGVESQGMLLCASIEGINRQVEPLDPPAGSAPGEHVFVKGYEKGQPDEELKPKKKVFEKLQADFKISEECIAQWKQTNFMTKLGSISCKSLKGGNIS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 tyrosyl-tRNA synthetase
别名 YARS1, 242400E9, CMTDIC, TYRRS, YRS
计算分子量 59 kDa
观测分子量60 kDa
GenBank蛋白编号BC016689
基因名称 YARS
Gene ID (NCBI) 8565
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP54577
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Tyrosyl-tRNA synthetase (YARS) is responsible for tyrosyl-tRNA aminoacylation. The functional enzyme exists as a homodimer, has potent leukocyte and monocyte chemotaxis activity, and stimulates production of myeloperoxidase, tumor necrosis factor-α, and tissue factor. A shift in the monomer:dimer equilibrium could result in dysregulation of the cytokines function. (PMID: 33490854)

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