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验证数据展示

  • WB analysis using 84859-3-RR

    WB analysis using 84859-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84859-3-RR (YARS antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 84859-3-RR

    IHC staining of human ovary cancer using 84859-3-RR

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 84859-3-RR (YARS antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of U2OS using 84859-3-RR

    IF Staining of U2OS using 84859-3-RR

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using YARS antibody (84859-3-RR, Clone: 242400E9 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 84859-3-RR

    Affinity and Kinetic Characterization of 84859-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84859-3-RR against Human YARS were performed. The affinity constant is 0.414 nM.

YARS Recombinant monoclonal antibody

YARS Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 84859-3-RR

产品说明书

CloneNo. 242400E9

宿主/亚型

Rabbit / IgG

种属反应性

human, pig

应用

WB, IHC, IF/ICC, ELISA

别名

YARS1, YTS, YRS, TYRRS, CMTDIC

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA431 cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84859-3-RR targets YARS in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, pig samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, pig
免疫原

CatNo: Ag35148

Product name: Recombinant human YARS protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 400-528 aa of BC016689

Sequence: RTVVSGLVQFVPKEELQDRLVVVLCNLKPQKMRGVESQGMLLCASIEGINRQVEPLDPPAGSAPGEHVFVKGYEKGQPDEELKPKKKVFEKLQADFKISEECIAQWKQTNFMTKLGSISCKSLKGGNIS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 tyrosyl-tRNA synthetase
别名 YARS1, YTS, YRS, TYRRS, CMTDIC
计算分子量 59 kDa
观测分子量60 kDa
GenBank蛋白编号BC016689
基因名称 YARS
Gene ID (NCBI) 8565
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP54577
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Tyrosyl-tRNA synthetase (YARS) is responsible for tyrosyl-tRNA aminoacylation. The functional enzyme exists as a homodimer, has potent leukocyte and monocyte chemotaxis activity, and stimulates production of myeloperoxidase, tumor necrosis factor-α, and tissue factor. A shift in the monomer:dimer equilibrium could result in dysregulation of the cytokines function. (PMID: 33490854)

实验方案

Product Specific Protocols
IF protocol for YARS antibody 84859-3-RRDownload protocol
IHC protocol for YARS antibody 84859-3-RRDownload protocol
WB protocol for YARS antibody 84859-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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