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验证数据展示

  • WB analysis of SK-BR-3 using 84852-4-RR

    WB analysis of SK-BR-3 using 84852-4-RR

    SK-BR-3 cells were subjected to SDS PAGE followed by western blot with 84852-4-RR (SUSD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of human placenta using 84852-4-RR

    WB analysis of human placenta using 84852-4-RR

    human placenta tissue were subjected to SDS PAGE followed by western blot with 84852-4-RR (SUSD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of SK-BR-3 using 84852-4-RR

    IF Staining of SK-BR-3 using 84852-4-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed SK-BR-3 cells using SUSD2 antibody (84852-4-RR, Clone: 242328A8 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

SUSD2 Recombinant monoclonal antibody

SUSD2 Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 84852-4-RR

产品说明书

CloneNo. 242328A8

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

242328A8, BK65A6.2, sushi domain containing 2

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inSK-BR-3 cells, human placenta tissue
Positive IF/ICC detected inSK-BR-3 cells
Planning an IHC experiment? We recommend our IHCeasy® SUSD2 Ready-To-Use IHC Kit. SUSD2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84852-4-RR targets SUSD2 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Eg2627

Product name: Recombinant Human SUSD2 protein (rFc Tag)

Source: mammalian cells-derived, V37

Tag: C-rFc

Domain: 28-782 aa of NM_019601.3

Sequence: QESCSMRCGALDGPCSCHPTCSGLGTCCLDFRDFCLEILPYSGSMMGGKDFVVRHFKMSSPTDASVICRFKDSIQTLGHVDSSGQVHCVSPLLYESGRIPFTVSLDNGHSFPRAGTWLAVHPNKVSMMEKSELVNETRWQYYGTANTSGNLSLTWHVKSLPTQTITIELWGYEETGMPYSQEWTAKWSYLYPLATHIPNSGSFTFTPKPAPPSYQRWRVGALRIIDSKNYAGQKDVQALWTNDHALAWHLSDDFREDPVAWARTQCQAWEELEDQLPNFLEELPDCPCTLTQARADSGRFFTDYGCDMEQGSVCTYHPGAVHCVRSVQASLRYGSGQQCCYTADGTQLLTADSSGGSTPDRGHDWGAPPFRTPPRVPSMSHWLYDVLSFYYCCLWAPDCPRYMQRRPSNDCRNYRPPRLASAFGDPHFVTFDGTNFTFNGRGEYVLLEAALTDLRVQARAQPGTMSNGTETRGTGLTAVAVQEGNSDVVEVRLANRTGGLEVLLNQEVLSFTEQSWMDLKGMFLSVAAGDRVSIMLASGAGLEVSVQGPFLSVSVLLPEKFLTHTHGLLGTLNNDPTDDFTLHSGRVLPPGTSPQELFLFGANWTVHNASSLLTYDSWFLVHNFLYQPKHDPTFEPLFPSETTLNPSLAQEAAKLCGDDHFCNFDVAATGSLSTGTATRVAHQLHQRRMQSLQPVVSCGWLAPPPNGQKEGNRYLAGSTIYFHCDNGYSLAGAETSTCQADGTWSSPTPKCQPGR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 sushi domain containing 2
别名 242328A8, BK65A6.2, sushi domain containing 2
计算分子量90 kDa
观测分子量60 kDa, 90 kDa, 110 kDa
GenBank蛋白编号NM_019601.3
基因名称 SUSD2
Gene ID (NCBI) 56241
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ9UGT4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Sushi domain containing 2 (SUSD2) is a type I transmembrane protein that contains a somatomedin B, AMOP, von Willebrand factor type D and Sushi domains, which are frequently found in molecules associated with cell-cell and cell-matrix adhesion. SUSD2 is composed of 822 amino acids with a predicted molecular weight of 90.4 kDa (PMID: 31387209). Three specific SUSD2 bands were present, the glycosylated, full-length protein (~110 kDa), ~90 kDa band represents full-length SUSD2 with minimal glycosylation, and a strong ~60 kDa band, represents a cleaved product of SUSD2 at the GD-PH sequence in the endoplasmic reticulum (PMID:27775699, 32595828).

实验方案

Product Specific Protocols
WB protocol for SUSD2 antibody 84852-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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