验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, HeLa cells, SGC-7901 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
84849-4-RR targets NUAK2 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag35302 Product name: Recombinant human NUAK2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 10005287 of NM_030952 Sequence: DPKEQKPPQASGLLLHRKGILKLNGKFSQTALELAAPTTFGSLDELAPPRPLARASRPSGAVSEDSILSSESFDQLDLPERLPEPPLRGCVSVDNLTGLEEPPSEGPGSCLRRWRQDPLGDSCFSLTDCQEVTATYRQALRVCSKLT 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | NUAK family, SNF1-like kinase, 2 |
| 别名 | OMPHK2, NUAK family SNF1-like kinase 2, NUAK 2, EC:2.7.11.1, ANPH2 |
| 计算分子量 | 70kDa |
| 观测分子量 | 70 kDa |
| GenBank蛋白编号 | NM_030952 |
| 基因名称 | NUAK2 |
| Gene ID (NCBI) | 81788 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purfication |
| UNIPROT ID | Q9H093 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NUAK2, also known as OMPHK2 and SNARK, is the fourth member of the AMPK kinase family. The molecular mass of NUAK2 is 69 kDa and it plays a key role in neural tube closure during embryonic development (PMID: 32845958).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NUAK2 antibody 84849-4-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
