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验证数据展示

  • WB analysis of HepG2 using 84810-4-RR

    WB analysis of HepG2 using 84810-4-RR

    HepG2 cells were subjected to SDS PAGE followed by western blot with 84810-4-RR (ACSS3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • WB analysis of THP-1 using 84810-4-RR

    WB analysis of THP-1 using 84810-4-RR

    THP-1 cells were subjected to SDS PAGE followed by western blot with 84810-4-RR (ACSS3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

ACSS3 Recombinant monoclonal antibody

ACSS3 Uni-rAb® Recombinant Antibody for WB, ELISA
Cat No. 84810-4-RR

产品说明书

CloneNo. 242423C6

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

EC:6.2.1.1, Acyl-CoA synthetase short-chain family member 3, mitochondrial, Acyl-CoA synthetase short-chain family member 3, ACSS 3, Acetate--CoA ligase 3

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHepG2 cells, THP-1 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:16000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84810-4-RR targets ACSS3 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag9173

Product name: Recombinant human ACSS3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 341-686 aa of BC009317

Sequence: DLGWVVGHSYICYGPLLHGNTTVLYEGKPVGTPDAGAYFRVLAEHGVAALFTAPTAIRAIRQQDPGAALGKQYSLTRFKTLFVAGERCDVETLEWSKNVFRVPVLDHWWQTETGSPITASCVGLGNSKTPPPGQAGKSVPGYNVMILDDNMQKLKARCLGNIVVKLPLPPGAFSGLWKNQEAFKHLYFEKFPGYYDTMDAGYMDEEGYLYVMSRVDDVINVAGHRISAGAIEESILSHGTVADCAVVGKEDPLKGHVPLALCVLRKDINATEEQVLEEIVKHVRQNIGPVAAFRNAVFVKQLPKTRSGKIPRSALSAIVNGKPYKITSTIEDPSIFGHVEEMLKQA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 acyl-CoA synthetase short-chain family member 3
别名 EC:6.2.1.1, Acyl-CoA synthetase short-chain family member 3, mitochondrial, Acyl-CoA synthetase short-chain family member 3, ACSS 3, Acetate--CoA ligase 3
计算分子量 686 aa, 75 kDa
观测分子量 70-75 kDa
GenBank蛋白编号BC009317
基因名称 ACSS3
Gene ID (NCBI) 79611
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ9H6R3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ACSS3(acyl-CoA synthetase short-chain family member 3, mitochondrial) belongs to the ATP-dependent AMP-binding enzyme family. It activates acetate so that it can be used for lipid synthesis or for energy generation. The deduced 686-amino acid protein contains 4 of 5 motifs characteristic of acyl-CoA synthetases. This protein has 2 isoforms produced by alternative splicing. The full length protein has a transit peptide with 29 amino acids.

实验方案

Product Specific Protocols
WB protocol for ACSS3 antibody 84810-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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