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验证数据展示

  • WB analysis using 84774-7-RR

    WB analysis using 84774-7-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84774-7-RR (ACSS2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • FC experiment of Caco-2 using 84774-7-RR

    FC experiment of Caco-2 using 84774-7-RR

    1x10^6 Caco-2 cells were intracellularly stained with 0.25 ug ACSS2 Recombinant antibody (84774-7-RR, Clone:242253C6) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • FC experiment of HepG2 using 84774-7-RR

    FC experiment of HepG2 using 84774-7-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug ACSS2 Recombinant antibody (84774-7-RR, Clone:242253C6) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • Affinity and Kinetic Characterization of 84774-7-RR

    Affinity and Kinetic Characterization of 84774-7-RR

    Biolayer interferometry (BLl) kinetic assays of 84774-7-RR against Human ACSS2 were performed. The affinity constant is 91.2 nM.

ACSS2 Recombinant monoclonal antibody

ACSS2 Uni-rAb® Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 84774-7-RR

产品说明书

CloneNo. 242253C6

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, FC (Intra), ELISA

别名

242253C6, ACAS2, AceCS1, Acetate--CoA ligase, Acetyl-CoA synthetase

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHepG2 cells, Caco-2 cells, mouse liver tissue, rat liver tissue, mouse brain tissue, rat brain tissue
Positive FC (Intra) detected inHepG2 cells, Caco-2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84774-7-RR targets ACSS2 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag9121

Product name: Recombinant human ACSS2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 185-534 aa of BC010141

Sequence: VFWCTADIGWITGHSYVTYGPLANGATSVLFEGIPTYPDVNRLWSIVDKYKVTKFYTAPTAIRLLMKFGDEPVTKHSRASLQVLGTVGEPINPEAWLWYHRVVGAQRCPIVDTFWQTETGGHMLTPLPGATPMKPGSATFPFFGVAPAILNESGEELEGEAEGYLVFKQPWPGIMRTVYGNHERFETTYFKKFPGYYVTGDGCQRDQDGYYWITGRIDDMLNVSGHLLSTAEVESALVEHEAVAEAAVVGHPHPVKGECLYCFVTLCDGHTFSPKLTEELKKQIREKIGPIATPDYIQNAPGLPKTRSGKIMRRVLRKIAQNDHDLGDMSTVADPSVISHLFSHRCLTIQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 acyl-CoA synthetase short-chain family member 2
别名 242253C6, ACAS2, AceCS1, Acetate--CoA ligase, Acetyl-CoA synthetase
计算分子量 701 aa, 79 kDa
观测分子量 79 kDa
GenBank蛋白编号BC010141
基因名称 ACSS2
Gene ID (NCBI) 55902
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ9NR19
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Acetyl-CoA Synthetase 2 (ACSS2) is also named as acyl-CoA synthetase short-chain family member 2. Acetyl-CoA Synthetase 2 (ACSS2) is responsible for lipogenesis by synthesizing acetyl-CoA from acetate for lipogenesis and promotes acetylation in nuclear (PMID: 29444517). It is also required for cell migration and invasion in renal cell carcinoma (RCC) through the promotion of autophagy (PMID: 29281714).

实验方案

Product Specific Protocols
FC protocol for ACSS2 antibody 84774-7-RRDownload protocol
WB protocol for ACSS2 antibody 84774-7-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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