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验证数据展示

  • WB analysis using 84765-4-RR

    WB analysis using 84765-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84765-4-RR (PDIA2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84765-4-RR

    Affinity and Kinetic Characterization of 84765-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84765-4-RR against Human PDIA2 were performed. The affinity constant is 24.0 pM.

PDIA2 Recombinant antibody

PDIA2 Recombinant Antibody for WB, ELISA
Cat No. 84765-4-RR

产品说明书

CloneNo. 242294E10

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, ELISA

别名

Protein disulfide isomerase A2, PDIR, PDIP, PDI, PDA2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse pancreas tissue, rat pancreas tissue, mouse stomach tissue, rat stomach tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84765-4-RR targets PDIA2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 PDIA2 fusion protein Ag35301 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 protein disulfide isomerase family A, member 2
别名 Protein disulfide isomerase A2, PDIR, PDIP, PDI, PDA2
计算分子量58 kDa
观测分子量60-65 kDa
GenBank蛋白编号NM_006849.2
基因名称 PDIA2
Gene ID (NCBI) 64714
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ13087
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PDIA2 (Protein disulfide-isomerase A2), a member of PDI family, is an ER protein chaperone that catalyzes the formation and breakage of disulfide bonds between cysteine residues and can inhibit aggregation of misfolded proteins by catalyzing rearrangement of -S-S- bonds in proteins. (PMID: 23486466)

实验方案

Product Specific Protocols
WB protocol for PDIA2 antibody 84765-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols