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验证数据展示

  • WB analysis of Transfected HEK-293T using 84734-3-RR

    WB analysis of Transfected HEK-293T using 84734-3-RR

    Human VEGFB Transfected HEK-293T cells were subjected to SDS PAGE followed by western blot with 84734-3-RR (VEGFB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of Transfected CHO-K1 using 84734-3-RR

    IF Staining of Transfected CHO-K1 using 84734-3-RR

    Immunofluorescent analysis of (4% PFA) fixed Transfected CHO-K1 cells using VEGFB antibody (84734-3-RR, Clone: 242024C10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 84734-3-RR

    Affinity and Kinetic Characterization of 84734-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84734-3-RR against Human VEGFB were performed. The affinity constant is 4.28 nM.

VEGFB Recombinant monoclonal antibody

VEGFB Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 84734-3-RR

产品说明书

CloneNo. 242024C10

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

242024C10, VEGF B, VEGF related factor, VEGF-B, VEGF-related factor

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHuman VEGFB Transfected HEK-293T cells
Positive IF/ICC detected inTransfected CHO-K1

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84734-3-RR targets VEGFB in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Eg2458

Product name: Recombinant Human VEGFB protein (rFc Tag)

Source: mammalian cells-derived, pHZ-KIsec-N-rFc

Tag: N-rFc

Domain: 22-207 aa of BC008818

Sequence: PVSQPDAPGHQRKVVSWIDVYTRATCQPREVVVPLTVELMGTVAKQLVPSCVTVQRCGGCCPDDGLECVPTGQHQVRMQILMIRYPSSQLGEMSLEEHSQCECRPKKKDSAVKPDRAATPHHRPQPRSVPGWDSAPGAPSPADITHPTPAPGPSAHAAPSTTSALTPGPAAAAADAAASSVAKGGA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 vascular endothelial growth factor B
别名 242024C10, VEGF B, VEGF related factor, VEGF-B, VEGF-related factor
计算分子量 22 kDa
观测分子量 22-30 kDa
GenBank蛋白编号BC008818
基因名称 VEGFB
Gene ID (NCBI) 7423
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDP49765
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Vascular endothelial growth factor B (VEGFB) is the third vascular endothelial growth factor family member to be discovered in 1996 . It has been demonstrated that vascular endothelial growth factor B (VEGFB) plays a vital role in regulating vascular biological function. VEGFB binds to vascular endothelial growth factor receptor 1 (VEGFR1) and neuropilin 1 (NRP1), which activates a lot of downstream activators similar to most tyrosine kinase receptors such as p38 MAPK, ERK/MAPK, PKB/AKT, and PI3K.(PMID: 34948148, PMID: 37375853)

实验方案

Product Specific Protocols
IF protocol for VEGFB antibody 84734-3-RRDownload protocol
WB protocol for VEGFB antibody 84734-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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