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验证数据展示

  • WB analysis using 84706-4-RR

    WB analysis using 84706-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84706-4-RR (IBTK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse lung using 84706-4-RR

    IHC staining of mouse lung using 84706-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 84706-4-RR (IBTK antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

IBTK Recombinant antibody

IBTK Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA
Cat No. 84706-4-RR

产品说明书

CloneNo. 242177D1

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

RP1 93K22.1, KIAA1417, BTKI, BTBD26, 242177D1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, HepG2 cells, Jurkat cells, Ramos cells
Positive IHC detected inmouse lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84706-4-RR targets IBTK in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 IBTK fusion protein Ag35421 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 inhibitor of Bruton agammaglobulinemia tyrosine kinase
别名 RP1 93K22.1, KIAA1417, BTKI, BTBD26, 242177D1
观测分子量160 kDa
GenBank蛋白编号BC143745
基因名称 IBTK
Gene ID (NCBI) 25998
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ9P2D0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IBTK, also known as BTKI and KIAA1417, which is involved in stress response and tumor growth. It has 3 isoforms and has been found to be hyper-expressed in chronic lymphocytic leukemia and correlated with disease progression (PMID: 29317636).

实验方案

Product Specific Protocols
WB protocol for IBTK antibody 84706-4-RRDownload protocol
IHC protocol for IBTK antibody 84706-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols