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验证数据展示

  • IHC staining of mouse brain using 84642-1-RR

    IHC staining of mouse brain using 84642-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84642-1-RR (UNC79 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 84642-1-RR

    IHC staining of mouse brain using 84642-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84642-1-RR (UNC79 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of SH-SY5Y using 84642-1-RR

    IF Staining of SH-SY5Y using 84642-1-RR

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using UNC79 antibody (84642-1-RR, Clone: 241628A7 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 84642-1-RR

    Affinity and Kinetic Characterization of 84642-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84642-1-RR against Human UNC79 were performed. The affinity constant is 11 nM.

UNC79 Recombinant antibody

UNC79 Recombinant Antibody for IHC, IF/ICC, ELISA
Cat No. 84642-1-RR

产品说明书

CloneNo. 241628A7

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IHC, IF/ICC, ELISA

别名

Protein unc-79 homolog, KIAA1409, 241628A7

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSH-SY5Y cells

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84642-1-RR targets UNC79 in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 UNC79 fusion protein Ag35440 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 KIAA1409
别名 Protein unc-79 homolog, KIAA1409, 241628A7
计算分子量295 kDa
GenBank蛋白编号NM_020818.4
基因名称 UNC79
Gene ID (NCBI) 57578
RRIDAB_3672101
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ9P2D8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

UNC79 is one of the key auxiliary subunits of the NALCN channel complex. Together with NALCN, FAM155A, and UNC80, it constitutes the NALCN channel body and is essential for maintaining neuronal excitability, motor function, pain sensitivity, and circadian rhythm (PMID: 35387979).

实验方案

Product Specific Protocols
IHC protocol for UNC79 antibody 84642-1-RRDownload protocol
IF protocol for UNC79 antibody 84642-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols