Urine of six individual healthy human donors was measured. The human KIM-1/HAVCR1 concentration of detected samples was determined to be 1,248.6 pg/mL with a range of 134.3-2,318.7 pg/mL.

A549 were cultured in DMEM supplemented with 10% fetal bovine serum, 2.5 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernate was removed, assayed for human KIM-1/HAVCR1, and measured 5,031.4 pg/mL.

Serum of eight individual healthy human donors was measured. The human KIM-1/HAVCR1 concentration of detected samples was determined to be 100.6 pg/mL with a range of 44.6-162.5 pg/mL.

Sandwich ELISA standard curve of MP01449-1, Human KIM-1/HAVCR1 Recombinant Matched Antibody Pair - PBS only. 84600-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1442. 84600-2-PBS was HRP conjugated as the detection antibody. Range: 9.77-625 pg/mL

Cytometric bead array standard curve of MP01449-1, KIM-1/HAVCR1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84600-1-PBS. Detection antibody: 84600-2-PBS. Standard: Eg1442. Range: 0.625-80 ng/mL.

Biolayer interferometry (BLl) kinetic assays of 84600-1-PBS against Human TIM-1/KIM-1/HAVCR were performed. The affinity constant is 1.29 nM.