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验证数据展示

  • WB analysis using 84418-7-RR

    WB analysis using 84418-7-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84418-7-RR (ZNHIT2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84418-7-RR

    Affinity and Kinetic Characterization of 84418-7-RR

    Biolayer interferometry (BLl) kinetic assays of 84418-7-RR against Human ZNHIT2 were performed. The affinity constant is below 1 pM.

ZNHIT2 Recombinant monoclonal antibody

ZNHIT2 Recombinant Antibody for WB, ELISA
Cat No. 84418-7-RR

产品说明书

CloneNo. 241868B10

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

ZNHIT 2, Zinc finger HIT domain-containing protein 2, FON, C11orf5, 241868B10

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293T cells, K-562 cells, HeLa cells, A549 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84418-7-RR targets ZNHIT2 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag10351

Product name: Recombinant human ZNHIT2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-403 aa of BC052240

Sequence: MEPAGPCGFCPAGEVQPARYTCPRCNAPYCSLRCYRTHGTCAENFYRDQVLGELRGCSAPPSRLASALRRLRQQRETEDEPGEAGLSSGPAPGGLSGLWERLAPGEKAAFERLLSRGEAGRLLPPWRPWWWNRGAGPQLLEELDNAPGSDAAELELAPARTPPDSVKDASAAEPAAAERVLGDVPGACTPVVPTRIPAIVSLSRGPVSPLVRFQLPNVLFAYAHTLALYHGGDDALLSDFCATLLGVSGALGAQQVFASAEEALQAAAHVLEAGEHPPGPLGTRGAMHEVARILLGEGPTNQKGYTLAALGDLAQTLGRARKQAVAREERDHLYRARKKCQFLLAWTNENEAALTPLALDCARAHQAHAVVAEEVAALTGELERLWGGPVPPAPRTLIEELPS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 zinc finger, HIT type 2
别名 ZNHIT 2, Zinc finger HIT domain-containing protein 2, FON, C11orf5, 241868B10
计算分子量 403 aa, 43 kDa
观测分子量 45-50 kDa
GenBank蛋白编号BC052240
基因名称 ZNHIT2
Gene ID (NCBI) 741
RRIDAB_3671947
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ9UHR6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ZNHIT2 belongs to a family of HIT-finger proteins characterized by the presence of a HIT domain, a roughly 50-residue zinc-binding domain containing conserved cysteine (Cys) and histidine (His) residues. ZNHIT2 is a cofactor of R2TP/PFDL that targets the U5 snRNP. ZNHIT2 is highly expressed in testis.

实验方案

Product Specific Protocols
WB protocol for ZNHIT2 antibody 84418-7-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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