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验证数据展示

  • WB analysis using 84375-3-RR

    WB analysis using 84375-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84375-3-RR (LINGO1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

LINGO1 Recombinant monoclonal antibody

LINGO1 Recombinant Antibody for WB, ELISA
Cat No. 84375-3-RR

产品说明书

CloneNo. 241599A9

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, ELISA

别名

Leucine-rich repeat neuronal protein 6A, Leucine-rich repeat neuronal protein 1, Leucine-rich repeat and immunoglobulin-like domain-containing nogo receptor-interacting protein 1, Leucine-rich repeat and immunoglobulin domain-containing protein 1, LERN1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue, fetal human brain tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84375-3-RR targets LINGO1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag13597

Product name: Recombinant human LINGO1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 36-351 aa of BC011057

Sequence: SGSATGCPPRCECSAQDRAVLCHRKRFVAVPEGIPTETRLLDLGKNRIKTLNQDEFASFPHLEELELNENIVSAVEPGAFNNLFNLRTLGLRSNRLKLIPLGVFTGLSNLTKLDISENKIVILLDYMFQDLYNLKSLEVGDNDLVYISHRAFSGLNSLEQLTLEKCNLTSIPTEALSHLHGLIVLRLRHLNINAIRDYSFKRLYRLKVLEISHWPYLDTMTPNCLYGLNLTSLSITHCNLTAVPYLAVRHLVYLRFLNLSYNPISTIEGSMLHELLRLQEIQLVGGQLAVVEPYAFRGLNYLRVLNVSGNQLTTLE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 leucine rich repeat and Ig domain containing 1
别名 Leucine-rich repeat neuronal protein 6A, Leucine-rich repeat neuronal protein 1, Leucine-rich repeat and immunoglobulin-like domain-containing nogo receptor-interacting protein 1, Leucine-rich repeat and immunoglobulin domain-containing protein 1, LERN1
计算分子量 70 kDa
观测分子量83 kDa
GenBank蛋白编号BC011057
基因名称 LINGO1
Gene ID (NCBI) 84894
RRIDAB_3671911
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ96FE5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LINGO-1 is a transmembrane receptor that contains 12 leucine-rich repeats and an immunoglobulin domain. It is expressed primarily in the central nervous system. LINGO-1 binds Nogo-66 receptor (NgR) and p75 and that is a functional component of the NgR/p75 signaling complex (PMID: 14966521). This protein has a calculated molecular weight of 70 kDa and migrates at higher molecular weight due to glycosylation (PMID: 21872576; 25257685; 18326721).

实验方案

Product Specific Protocols
WB protocol for LINGO1 antibody 84375-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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