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验证数据展示

  • WB analysis using 84284-5-RR

    WB analysis using 84284-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84284-5-RR (TMEM98 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 84284-5-RR

    WB analysis of HepG2 using 84284-5-RR

    WB result of TMEM98 antibody (84284-5-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TMEM98 transfected HepG2 cells.

  • Affinity and Kinetic Characterization of 84284-5-RR

    Affinity and Kinetic Characterization of 84284-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84284-5-RR against Human TMEM98 were performed. The affinity constant is below 1 pM.

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  • KD/KO验证

TMEM98 Recombinant monoclonal antibody

TMEM98 Uni-rAb® Recombinant Antibody for WB, ELISA
Cat No. 84284-5-RR

产品说明书

CloneNo. 241581A6

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

241581A6, Protein TADA1, Transmembrane protein 98, UNQ536/PRO1079

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA549 cells, HepG2 cells, Y79 cells, NCCIT cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84284-5-RR targets TMEM98 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag6492

Product name: Recombinant human TMEM98 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 27-226 aa of BC000526

Sequence: CRQRYCRPRDLLQRYDSKPIVDLIGAMETQSEPSELELDDVVITNPHIEAILENEDWIEDASGLMSHCIAILKICHTLTEKLVAMTMGSGAKMKTSASVSDIIVVAKRISPRVDDVVKSMYPPLDPKLLDARTTALLLSVSHLVLVTRNACHLTGGLDWIDQSLSAAEEHLEVLREAALASEPDKGLPGPEGFLQEQSAI

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 transmembrane protein 98
别名 241581A6, Protein TADA1, Transmembrane protein 98, UNQ536/PRO1079
计算分子量 25 kDa
观测分子量25 kDa
GenBank蛋白编号BC000526
基因名称 TMEM98
Gene ID (NCBI) 26022
RRIDAB_3671831
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ9Y2Y6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TMEM98, also named as TADA1, composed of 226 amino acid residues, is a potential single-pass transmembrane protein. It is expression in both nucleus and cytoplasm. TMEM98 was found to be over-expressed in most of the hepatocellular carcinoma tissue. MEM98 is not only a prognostic marker for patients with hepatocellular carcinoma but also a novel molecular target associated with intrinsic and acquired chemoresistance of hepatocellular carcinoma (PMID: 24608572).

实验方案

Product Specific Protocols
WB protocol for TMEM98 antibody 84284-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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