×
Use Able AI chat for product recommendations

验证数据展示

  • WB analysis of Raji using 84181-1-RR

    WB analysis of Raji using 84181-1-RR

    Raji cells were subjected to SDS PAGE followed by western blot with 84181-1-RR (Neprilysin/CD10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 84181-1-RR

    WB analysis using 84181-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84181-1-RR (Neprilysin/CD10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 84181-1-RR

    IHC staining of human tonsillitis using 84181-1-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84181-1-RR (Neprilysin/CD10 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD10 antibody included
  • IHC staining of human placenta using 84181-1-RR

    IHC staining of human placenta using 84181-1-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84181-1-RR (Neprilysin/CD10 antibody) at dilution of 1:1000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • IHC staining of human pancreas cancer using 84181-1-RR

    IHC staining of human pancreas cancer using 84181-1-RR

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 84181-1-RR (Neprilysin/CD10 antibody) at dilution of 1:1000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • IHC staining of human renal cell carcinoma using 84181-1-RR

    IHC staining of human renal cell carcinoma using 84181-1-RR

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 84181-1-RR (Neprilysin/CD10 antibody) at dilution of 1:1000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • IHC staining of human renal cell carcinoma using 84181-1-RR

    IHC staining of human renal cell carcinoma using 84181-1-RR

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 84181-1-RR (Neprilysin/CD10 antibody) at dilution of 1:1000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • IHC staining of human lymphoma using 84181-1-RR

    IHC staining of human lymphoma using 84181-1-RR

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 84181-1-RR (Neprilysin/CD10 antibody) at dilution of 1:1000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • IHC staining of human lymphoma using 84181-1-RR

    IHC staining of human lymphoma using 84181-1-RR

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 84181-1-RR (Neprilysin/CD10 antibody) at dilution of 1:1000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • IHC staining of human tonsillitis using 84181-1-RR

    IHC staining of human tonsillitis using 84181-1-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84181-1-RR (Neprilysin/CD10 antibody) at dilution of 1:1000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • IHC staining of human liver using 84181-1-RR

    IHC staining of human liver using 84181-1-RR

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 84181-1-RR (Neprilysin/CD10 antibody) at dilution of 1:1000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • IHC staining of human appendicitis using 84181-1-RR

    IHC staining of human appendicitis using 84181-1-RR

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 84181-1-RR (Neprilysin/CD10 antibody) at dilution of 1:1000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • IHC staining of human kidney using 84181-1-RR

    IHC staining of human kidney using 84181-1-RR

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 84181-1-RR (Neprilysin/CD10 antibody) at dilution of 1:1000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • IHC staining of human kidney using 84181-1-RR

    IHC staining of human kidney using 84181-1-RR

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 84181-1-RR (Neprilysin/CD10 antibody) at dilution of 1:1000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD10 antibody included
  • IHC staining of human tonsillitis using 84181-1-RR

    IHC staining of human tonsillitis using 84181-1-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84181-1-RR (Neprilysin/CD10 antibody) at dilution of 1:1000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD10 antibody included
  • IHC staining of human liver using 84181-1-RR

    IHC staining of human liver using 84181-1-RR

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 84181-1-RR (Neprilysin/CD10 antibody) at dilution of 1:1000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD10 antibody included
  • IHC staining of human renal cell carcinoma using 84181-1-RR

    IHC staining of human renal cell carcinoma using 84181-1-RR

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 84181-1-RR (Neprilysin/CD10 antibody) at dilution of 1:1000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD10 antibody included
  • IHC staining of human lymphoma using 84181-1-RR

    IHC staining of human lymphoma using 84181-1-RR

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 84181-1-RR (Neprilysin/CD10 antibody) at dilution of 1:1000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD10 antibody included
  • IHC staining of human pancreas cancer using 84181-1-RR

    IHC staining of human pancreas cancer using 84181-1-RR

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 84181-1-RR (Neprilysin/CD10 antibody) at dilution of 1:1000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD10 antibody included
  • IHC staining of human renal cell carcinoma using 84181-1-RR

    IHC staining of human renal cell carcinoma using 84181-1-RR

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 84181-1-RR (Neprilysin/CD10 antibody) at dilution of 1:1000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD10 antibody included
  • IHC staining of human placenta using 84181-1-RR

    IHC staining of human placenta using 84181-1-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84181-1-RR (Neprilysin/CD10 antibody) at dilution of 1:1000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD10 antibody included
  • IF Staining of mouse kidney using 84181-1-RR

    IF Staining of mouse kidney using 84181-1-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using Neprilysin/CD10 antibody (84181-1-RR, Clone: 241322A8 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 84181-1-RR

    Affinity and Kinetic Characterization of 84181-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84181-1-RR against Human Neprilysin/CD10 were performed. The affinity constant is 3.07 nM.

Neprilysin/CD10 Recombinant monoclonal antibody

Neprilysin/CD10 Uni-rAb® Recombinant Antibody for WB, IHC, IF-P, ELISA
Cat No. 84181-1-RR

产品说明书

CloneNo. 241322A8

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, ELISA

别名

MME, Neprilysin, 241322A8, Atriopeptidase, CALLA

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inmouse kidney tissue, Raji cells, rat kidney tissue
Positive IHC detected inhuman tonsillitis tissue, human lymphoma tissue, human kidney tissue, human placenta tissue, human renal cell carcinoma tissue, human pancreas cancer tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse kidney tissue
Planning an IHC experiment? We recommend our IHCeasy CD10 Ready-To-Use IHC Kit. CD10 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84181-1-RR targets Neprilysin/CD10 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

Recombinant Protein

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 membrane metallo-endopeptidase
别名 MME, Neprilysin, 241322A8, Atriopeptidase, CALLA
计算分子量 750 aa, 86 kDa
观测分子量 100 kDa
GenBank蛋白编号BC101658
基因名称 CD10
Gene ID (NCBI) 4311
RRIDAB_3671736
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP08473
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD10, also known as neprilysin, membrane metallo-endopeptidase (MME), neutral endopeptidase (NEP), or common acute lymphoblastic leukemia antigen (CALLA), is a 100-kDa type II transmembrane glycoprotein belonging to peptidase M13 family (PMID: 7760013; 8102558). Among hematopoietic cells, CD10 is expressed on granulocytes, B cell precursors, mature germinal center B cells, a subset of immature thymocytes (PMID: 13679451). CD10 is also expressed on a variety of nonhematopoietic cell types, including bronchial epithelial cells, cultured fibroblasts, bone marrow stromal cells, renal proximal tubular epithelial cells, breast myoepithelium, biliary canaliculi (PMID: 8102558). CD10 is a cell surface peptidase that cleaves peptide bonds on the amino side of hydrophobic amino acids and inactivates a variety of physiologically active peptides. Loss or decreases in CD10 expression have been reported in a variety of malignancies (PMID: 16054017).

实验方案

Product Specific Protocols
IF protocol for Neprilysin/CD10 antibody 84181-1-RRDownload protocol
IHC protocol for Neprilysin/CD10 antibody 84181-1-RRDownload protocol
WB protocol for Neprilysin/CD10 antibody 84181-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
||
New chat

Able™

正在加载，请稍候...