验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, MCF-7 cells, K-562 cells, U2OS cells, U-521 cells, MDA-MB-231 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
84167-3-RR targets TRMT12 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag9258 Product name: Recombinant human TRMT12 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 99-448 aa of BC011713 Sequence: QGCSPAQKLCLEVSRWVEGRGVKWSAELEADLPRSWQRHGNLLLLSEDCFQAKQWKNLGPELWETVALALGVQRLAKRGRVSPDGTRTPAVTLLLGDHGWVEHVDNGIRYKFDVTQCMFSFGNITEKLRVASLSCAGEVLVDLYAGIGYFTLPFLVHAGAAFVHACEWNPHAVVALRNNLEINGVADRCQIHFGDNRKLKLSNIADRVILGLIPSSEEGWPIACQVLRQDAGGILHIHQNVESFPGKNLQALGVSKVEKEHWLYPQQITTNQWKNGATRDSRGKMLSPATKPEWQRWAESAETRIATLLQQVHGKPWKTQILHIQPVKSYAPHVDHIVLDLECCPCPSVG 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | tRNA methyltransferase 12 homolog (S. cerevisiae) |
| 别名 | 241483H1, EC:2.5.1.114, TRM12, tRNA methyltransferase 12, tRNA wybutosine-synthesizing protein 2 homolog |
| 计算分子量 | 448 aa, 50 kDa |
| 观测分子量 | 45-50 kDa |
| GenBank蛋白编号 | BC011713 |
| 基因名称 | TRMT12 |
| Gene ID (NCBI) | 55039 |
| RRID | AB_3671727 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purfication |
| UNIPROT ID | Q53H54 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TRMT12 antibody 84167-3-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
