验证数据展示
经过测试的应用
| Positive WB detected in | Y79 cells, mouse retina tissue, rat retina tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
84163-3-RR targets ARL4D in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | ARL4D fusion protein Ag7369 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | ADP-ribosylation factor-like 4D |
| 别名 | ARF4L, ARL6, ADP-ribosylation factor-like protein 4L, ADP-ribosylation factor-like protein 4D, 241503B7 |
| 计算分子量 | 22 kDa |
| 观测分子量 | 25-27 kDa |
| GenBank蛋白编号 | BC000043 |
| 基因名称 | ARL4D |
| Gene ID (NCBI) | 379 |
| RRID | AB_3671725 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purfication |
| UNIPROT ID | P49703 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ARL4D (ADP-ribosylation factor-like protein 4D) is also named as ARF4L. ARL4D is a developmentally regulated member of the ADP-ribosylation factor/ARF-like protein (ARF/ARL) family of Ras-related GTPases. ARL4D interacts with the C-terminal pleckstrin homology (PH) and polybasic c domains of cytohesin-2/ARNO in a GTP-dependent manner. Localization of ARL4D at the plasma membrane is GTP- and N-terminal myristoylation-dependent. ARL4D(Q80L), a putative active form of ARL4D, induced accumulation of cytohesin-2/ARNO at the plasma membrane (PMID: 17804820).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ARL4D antibody 84163-3-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |