• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 84119-3-RR (same clone as 84119-3-PBS)

    WB analysis using 84119-3-RR (same clone as 84119-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84119-3-RR (TRAPPC4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84119-3-PBS in a different storage buffer formulation.

  • WB analysis of MCF-7 using 84119-3-RR (same clone as 84119-3-PBS)

    WB analysis of MCF-7 using 84119-3-RR (same clone as 84119-3-PBS)

    WB result of TRAPPC4 antibody (84119-3-RR; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TRAPPC4 transfected MCF-7 cells. This data was developed using the same antibody clone with 84119-3-PBS in a different storage buffer formulation.

  • IP experiment of HL-60 using 84119-3-RR (same clone as 84119-3-PBS)

    IP experiment of HL-60 using 84119-3-RR (same clone as 84119-3-PBS)

    IP result of anti-TRAPPC4 (IP:84119-3-RR, 4ug; Detection:84119-3-RR 1:3000) with HL-60 cells lysate 2240 ug. This data was developed using the same antibody clone with 84119-3-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 84119-3-RR (same clone as 84119-3-PBS)

    IF Staining of HeLa using 84119-3-RR (same clone as 84119-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TRAPPC4 antibody (84119-3-RR, Clone: 241158C11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84119-3-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01030-2

    Cytometric bead array standard curve of MP01030-2

    Cytometric bead array standard curve of MP01030-2, TRAPPC4 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84119-4-PBS. Detection antibody: 84119-3-PBS. Standard: Ag2737. Range: 0.781-100 ng/mL.

  • Cytometric bead array standard curve of MP01030-3

    Cytometric bead array standard curve of MP01030-3

    Cytometric bead array standard curve of MP01030-3, TRAPPC4 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84119-1-PBS. Detection antibody: 84119-3-PBS. Standard: Ag2737. Range: 0.781-100 ng/mL.

  • Affinity and Kinetic Characterization of 84119-3-RR

    Affinity and Kinetic Characterization of 84119-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84119-3-RR against Human TRAPPC4 were performed. The affinity constant is below 1 pM.

  • Featured Product
  • KD/KO验证

TRAPPC4 Recombinant antibody, PBS Only (Detector)

TRAPPC4 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, IP, Cytometric bead array, Indirect ELISA
Cat No. 84119-3-PBS

产品说明书

CloneNo. 241158C11

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, IP, Cytometric bead array, Indirect ELISA

别名

241158C11, CGI 104, CGI-104, HSPC172, PTD009

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

产品信息

84119-3-PBS targets TRAPPC4 as part of a matched antibody pair:

MP01030-2: 84119-4-PBS capture and 84119-3-PBS detection (validated in Cytometric bead array)

MP01030-3: 84119-1-PBS capture and 84119-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, IP, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 TRAPPC4 fusion protein Ag2737 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 trafficking protein particle complex 4
别名 241158C11, CGI 104, CGI-104, HSPC172, PTD009
计算分子量 219 aa, 24 kDa
观测分子量 24 kDa
GenBank蛋白编号BC010866
基因名称 TRAPPC4
Gene ID (NCBI) 51399
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9Y296
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Transport protein particle (TRAPP, also known as trafficking protein particle), a multimeric guanine nucleotide-exchange factor, regulates multiple membrane trafficking pathways (PMID: 20966969). TRAPPC4, also known as synbindin, is a core component of the TRAPP complexes and one of the essential subunits for guanine nucleotide exchange factor activity for Rab1 GTPase (PMID: 31794024). Deficiencies in vesicular transport mediated by TRAPPC4 have been associated with severe syndromic intellectual disability (PMID: 31794024).