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验证数据展示

  • WB analysis using 84033-3-RR

    WB analysis using 84033-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84033-3-RR (ACSF2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • FC experiment of A549 using 84033-3-RR

    FC experiment of A549 using 84033-3-RR

    1x10^6 A549 cells were intracellularly stained with 0.25 ug ACSF2 Recombinant antibody (84033-3-RR, Clone:241208D9) and Multi-rAb CoraLite ® Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR005)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • Affinity and Kinetic Characterization of 84033-3-RR

    Affinity and Kinetic Characterization of 84033-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84033-3-RR against Human ACSF2 were performed. The affinity constant is 1.94 nM.

ACSF2 Recombinant monoclonal antibody

ACSF2 Uni-rAb® Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 84033-3-RR

产品说明书

CloneNo. 241208D9

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), ELISA

别名

241208D9, ACSF 2, EC:6.2.1.2, Medium-chain acyl-CoA ligase ACSF2, mitochondrial, UNQ493/PRO1009

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inJurkat cells, SGC-7901 cells
Positive FC (Intra) detected inA549 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84033-3-RR targets ACSF2 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag9199

Product name: Recombinant human ACSF2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 336-615 aa of BC012053

Sequence: FNGKKALEAISRERGTFLYGTPTMFVDILNQPDFSSYDISTMCGGVIAGSPAPPELIRAIINKINMKDLVVAYGTTENSPVTFAHFPEDTVEQKAESVGRIMPHTEARIMNMEAGTLAKLNTPGELCIRGYCVMLGYWGEPQKTEEAVDQDKWYWTGDVATMNEQGFCKIVGRSKDMIIRGGENIYPAELEDFFHTHPKVQEVQVVGVKDDRMGEEICACIRLKDGEETTVEEIKAFCKGKISHFKIPKYIVFVTNYPLTISGKIQKFKLREQMERHLNL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 acyl-CoA synthetase family member 2
别名 241208D9, ACSF 2, EC:6.2.1.2, Medium-chain acyl-CoA ligase ACSF2, mitochondrial, UNQ493/PRO1009
计算分子量 615 aa, 68 kDa
观测分子量51-71 kDa
GenBank蛋白编号BC012053
基因名称 ACSF2
Gene ID (NCBI) 80221
RRIDAB_3671601
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ96CM8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ACSF2 (encoded by acyl-CoA synthetase family member 2) belongs to the acyl-CoA synthetase (ACS) family, activating fatty acids by forming a thioester bond with CoA.

实验方案

Product Specific Protocols
FC protocol for ACSF2 antibody 84033-3-RRDownload protocol
WB protocol for ACSF2 antibody 84033-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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