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验证数据展示

  • WB analysis using 84009-4-RR

    WB analysis using 84009-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84009-4-RR (CALCOCO1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of rat testis using 84009-4-RR

    IHC staining of rat testis using 84009-4-RR

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 84009-4-RR (CALCOCO1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 84009-4-RR

    IHC staining of mouse testis using 84009-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 84009-4-RR (CALCOCO1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 84009-4-RR

    Affinity and Kinetic Characterization of 84009-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84009-4-RR against Human CALCOCO1 were performed. The affinity constant is below 1 pM.

CALCOCO1 Recombinant monoclonal antibody

CALCOCO1 Recombinant Antibody for WB, IHC, ELISA
Cat No. 84009-4-RR

产品说明书

CloneNo. 241127G7

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

PP13275, Coiled-coil coactivator protein, Cocoa, Calphoglin, Calcium-binding and coiled-coil domain-containing protein 1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHT-1080 cells, MCF-7 cells, HeLa cells, HEK-293T cells, Ramos cells, rat brain tissue
Positive IHC detected inmouse testis tissue, rat testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84009-4-RR targets CALCOCO1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag13876

Product name: Recombinant human CALCOCO1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 341-691 aa of BC003177

Sequence: LKEQLRGAQELAASSQQKATLLGEELASAAAARDRTIAELHRSRLEVAEVNGRLAELGLHLKEEKCQWSKERAGLLQSVEAEKDKILKLSAEILRLEKAVQEERTQNQVFKTELAREKDSSLVQLSESKRELTELRSALRVLQKEKEQLQEEKQELLEYMRKLEARLEKVADEKWNEDATTEDEEAAVGLSCPAALTDSEDESPEDMRLPPYGLCERGDPGSSPAGPREASPLVVISQPAPISPHLSGPAEDSSSDSEAEDEKSVLMAAVQSGGEEANLLLPELGSAFYDMASGFTVGTLSETSTGGPATPTWKECPICKERFPAESDKDALEDHMDGHFFFSTQDPFTFE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 calcium binding and coiled-coil domain 1
别名 PP13275, Coiled-coil coactivator protein, Cocoa, Calphoglin, Calcium-binding and coiled-coil domain-containing protein 1
计算分子量 691 aa, 77 kDa
观测分子量100 kDa
GenBank蛋白编号BC003177
基因名称 CALCOCO1
Gene ID (NCBI) 57658
RRIDAB_3671577
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ9P1Z2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for CALCOCO1 antibody 84009-4-RRDownload protocol
WB protocol for CALCOCO1 antibody 84009-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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