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验证数据展示

  • WB analysis using 83991-4-RR

    WB analysis using 83991-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83991-4-RR (E-cadherin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 83991-4-RR

    IHC staining of human colon cancer using 83991-4-RR

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 83991-4-RR (E-cadherin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with E-cadherin antibody included
  • IHC staining of human colon cancer using 83991-4-RR

    IHC staining of human colon cancer using 83991-4-RR

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 83991-4-RR (E-cadherin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with E-cadherin antibody included
  • IF Staining of A431 using 83991-4-RR

    IF Staining of A431 using 83991-4-RR

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using E-cadherin antibody (83991-4-RR, Clone: 241098G6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • IHC staining of human liver using 83991-4-RR

    IHC staining of human liver using 83991-4-RR

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 83991-4-RR (E-cadherin antibody) at dilution of 1:1000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • IHC staining of human stomach cancer using 83991-4-RR

    IHC staining of human stomach cancer using 83991-4-RR

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 83991-4-RR (E-cadherin antibody) at dilution of 1:1000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • IHC staining of human colorectal cancer using 83991-4-RR

    IHC staining of human colorectal cancer using 83991-4-RR

    Immunohistochemical analysis of paraffin-embedded human colorectal cancer tissue slide using 83991-4-RR (E-cadherin antibody) at dilution of 1:1000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with E-cadherin antibody included
  • IHC staining of human breast cancer using 83991-4-RR

    IHC staining of human breast cancer using 83991-4-RR

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 83991-4-RR (E-cadherin antibody) at dilution of 1:1000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with E-cadherin antibody included
  • IHC staining of human breast cancer using 83991-4-RR

    IHC staining of human breast cancer using 83991-4-RR

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 83991-4-RR (E-cadherin antibody) at dilution of 1:1000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with E-cadherin antibody included
  • IHC staining of human ovary cancer using 83991-4-RR

    IHC staining of human ovary cancer using 83991-4-RR

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83991-4-RR (E-cadherin antibody) at dilution of 1:1000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with E-cadherin antibody included
  • IHC staining of human tonsillitis using 83991-4-RR

    IHC staining of human tonsillitis using 83991-4-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 83991-4-RR (E-cadherin antibody) at dilution of 1:1000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with E-cadherin antibody included
  • IHC staining of human liver using 83991-4-RR

    IHC staining of human liver using 83991-4-RR

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 83991-4-RR (E-cadherin antibody) at dilution of 1:1000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with E-cadherin antibody included
  • IHC staining of human colon cancer using 83991-4-RR

    IHC staining of human colon cancer using 83991-4-RR

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 83991-4-RR (E-cadherin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with E-cadherin antibody included
  • IHC staining of human placenta using 83991-4-RR

    IHC staining of human placenta using 83991-4-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83991-4-RR (E-cadherin antibody) at dilution of 1:1000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with E-cadherin antibody included
  • IHC staining of human appendicitis using 83991-4-RR

    IHC staining of human appendicitis using 83991-4-RR

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 83991-4-RR (E-cadherin antibody) at dilution of 1:1000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with E-cadherin antibody included
  • IHC staining of human stomach cancer using 83991-4-RR

    IHC staining of human stomach cancer using 83991-4-RR

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 83991-4-RR (E-cadherin antibody) at dilution of 1:1000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with E-cadherin antibody included
  • Affinity and Kinetic Characterization of 83991-4-RR

    Affinity and Kinetic Characterization of 83991-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83991-4-RR against Human E-cadherin were performed. The affinity constant is 2.78 nM.

E-cadherin Recombinant monoclonal antibody

E-cadherin Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 83991-4-RR

产品说明书

CloneNo. 241098G6
引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

241098G6, Arc 1, Cadherin 1, Cadherin-1, CAM 120/80

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inA431 cells, HepG2 cells, T-47D cells, HCT 116 cells
Positive IHC detected inhuman colon cancer tissue, human ovary cancer tissue, human tonsillitis tissue, human stomach cancer tissue, human appendicitis tissue, human breast cancer tissue, human colon cancer tissue, human placenta tissue, human colorectal cancer tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells, MCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy E-cadherin Ready-To-Use IHC Kit. E-cadherin primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

83991-4-RR targets E-cadherin in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性human
免疫原

CatNo: Eg1112

Product name: Recombinant Human E-cadherin protein (His Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: C-6*HIS

Domain: 155-709 aa of NM_004360.5

Sequence: DWVIPPISCPENEKGPFPKNLVQIKSNKDKEGKVFYSITGQGADTPPVGVFIIERETGWLKVTEPLDRERIATYTLFSHAVSSNGNAVEDPMEILITVTDQNDNKPEFTQEVFKGSVMEGALPGTSVMEVTATDADDDVNTYNAAIAYTILSQDPELPDKNMFTINRNTGVISVVTTGLDRESFPTYTLVVQAADLQGEGLSTTATAVITVTDTNDNPPIFNPTTYKGQVPENEANVVITTLKVTDADAPNTPAWEAVYTILNDDGGQFVVTTNPVNNDGILKTAKGLDFEAKQQYILHVAVTNVVPFEVSLTTSTATVTVDVLDVNEAPIFVPPEKRVEVSEDFGVGQEITSYTAQEPDTFMEQKITYRIWRDTANWLEINPDTGAISTRAELDREDFEHVKNSTYTALIIATDNGSPVATGTGTLLLILSDVNDNAPIPEPRTIFFCERNPKPQVINIIDADLPPNTSPFTAELTHGASANWTIQYNDPTQESIILKPKMALEVGDYKINLKLMDNQNKDQVTTLEVSVCDCEGAAGVCRKAQPVEAGLQIPA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 cadherin 1, type 1, E-cadherin (epithelial)
别名 241098G6, Arc 1, Cadherin 1, Cadherin-1, CAM 120/80
计算分子量97 kDa
观测分子量135 kDa
GenBank蛋白编号NM_004360.5
基因名称 E-cadherin
Gene ID (NCBI) 999
RRIDAB_3671566
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP12830-1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. E-cadherin (epithelial cadherin), also known as CDH1 (cadherin 1) or CAM 120/80, is a classical member of the cadherin superfamily which also include N-, P-, R-, and B-cadherins. E-cadherin is expressed on the cell surface in most epithelial tissues. The extracellular region of E-cadherin establishes calcium-dependent homophilic trans binding, providing specific interaction with adjacent cells, while the cytoplasmic domain is connected to the actin cytoskeleton through the interaction with p120-, α-, β-, and γ-catenin (plakoglobin). E-cadherin is important in the maintenance of the epithelial integrity, and is involved in mechanisms regulating proliferation, differentiation, and survival of epithelial cell. E-cadherin may also play a role in tumorigenesis. It is considered to be an invasion suppressor protein and its loss is an indicator of high tumor aggressiveness.

实验方案

Product Specific Protocols
IF protocol for E-cadherin antibody 83991-4-RRDownload protocol
IHC protocol for E-cadherin antibody 83991-4-RRDownload protocol
WB protocol for E-cadherin antibody 83991-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

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Authors - Xiaohu Guo
View Article
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