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验证数据展示

  • IF Staining of RAW 264.7 using 83988-4-RR

    IF Staining of RAW 264.7 using 83988-4-RR

    Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using GDF5 antibody (83988-4-RR, Clone: 240944E5 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 83988-4-RR

    Affinity and Kinetic Characterization of 83988-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83988-4-RR against Human GDF5 were performed. The affinity constant is 4.07 nM.

GDF5 Recombinant antibody

GDF5 Recombinant Antibody for IF/ICC, ELISA
Cat No. 83988-4-RR

产品说明书

CloneNo. 240944E5

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IF/ICC, ELISA

别名

GDF-5, GDF 5, CDMP-1, Cartilage-derived morphogenetic protein 1, Bone morphogenetic protein 14

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inRAW 264.7 cells

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83988-4-RR targets GDF5 in IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 GDF5 fusion protein Ag6238 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 growth differentiation factor 5
别名 GDF-5, GDF 5, CDMP-1, Cartilage-derived morphogenetic protein 1, Bone morphogenetic protein 14
计算分子量 55 kDa
GenBank蛋白编号BC032495
基因名称 GDF5
Gene ID (NCBI) 8200
RRIDAB_3671563
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP43026
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Growth and Differentiation Factor 5 (GDF5) plays important roles during joint formation. It is one of the earliest genes expressed in the embryonic joint interzone7-10, fated to give rise to joint tissues including articular cartilage, synovium, menisci, and ligaments.

实验方案

Product Specific Protocols
IF protocol for GDF5 antibody 83988-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols