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验证数据展示

  • WB analysis using 83803-1-RR

    WB analysis using 83803-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83803-1-RR (MITF antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • FC experiment of HeLa using 83803-1-RR

    FC experiment of HeLa using 83803-1-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug MITF Recombinant Antibody (83803-1-RR, Clone:240815B4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

MITF Recombinant monoclonal antibody

MITF Uni-rAb® Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 83803-1-RR

产品说明书

CloneNo. 240815B4

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), ELISA

别名

bHLHe32, Class E basic helix-loop-helix protein 32, MI, Microphthalmia-associated transcription factor, WS2A

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, Jurkat cells
Positive FC (Intra) detected inHeLa cells, SW480 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83803-1-RR targets MITF in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag3679

Product name: Recombinant human MITF protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-91 aa of BC012503

Sequence: MLEMLEYNHYQVQTHLENPTKYHIQQAQRQQVKQYLSTTLANKHANQVLSLPCPNQPGDHVMPPVPGSSAPNSPMAMLTLNSNCEKEFMKQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 microphthalmia-associated transcription factor
别名 bHLHe32, Class E basic helix-loop-helix protein 32, MI, Microphthalmia-associated transcription factor, WS2A
计算分子量 91 aa, 10 kDa, 59 kDa
观测分子量 60-65 kDa
GenBank蛋白编号BC012503
基因名称 MITF
Gene ID (NCBI) 4286
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO75030
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The retinal pigment epithelium (RPE) has a essential role in maintaining visual function and dedifferentiation of RPE contributes to the pathophysiology of several ocular diseases (PMID:22523078). Microphthalmia-associated transcription factor (MITF) is a key regulator of RPE differentiation that is also down-regulated in dedifferentiated hfRPE cells. MITF is a basic helix-loop-helix (hHLH)-leucine zipper protein that involves in the development of various cell types, including neural crest-derived melanocytes and optic cup-derived retinal pigment epithelial cells (PMID: 10578055).

实验方案

Product Specific Protocols
WB protocol for MITF antibody 83803-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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