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验证数据展示

  • WB analysis of rat lung using 83759-4-RR

    WB analysis of rat lung using 83759-4-RR

    rat lung tissue were subjected to SDS PAGE followed by western blot with 83759-4-RR (RAGE antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of rat brain using 83759-4-RR

    IF Staining of rat brain using 83759-4-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using AGER antibody (83759-4-RR, Clone: 240706F7 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of rat brain using 83759-4-RR

    IF Staining of rat brain using 83759-4-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using AGER antibody (83759-4-RR, Clone: 240706F7 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 83759-4-RR

    Affinity and Kinetic Characterization of 83759-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83759-4-RR against Mouse AGER were performed. The affinity constant is below 1 pM.

AGER/RAGE Recombinant antibody

AGER/RAGE Uni-rAbTM Recombinant Antibody for WB, IF-P, ELISA
Cat No. 83759-4-RR

产品说明书

CloneNo. 240706F7

宿主/亚型

Rabbit / IgG

种属反应性

rat

应用

WB, IF-P, ELISA

别名

RAGE, AGER, Advanced glycosylation end product-specific receptor, 240706F7

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inrat lung tissue
Positive IF-P detected inrat brain tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83759-4-RR targets AGER/RAGE in WB, IF-P, ELISA applications and shows reactivity with rat samples.

经测试应用 WB, IF-P, ELISA Application Description
经测试反应性 rat
免疫原 Fusion Protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 advanced glycosylation end product-specific receptor
别名 RAGE, AGER, Advanced glycosylation end product-specific receptor, 240706F7
计算分子量43 kDa
观测分子量45 kDa, 58 kDa
基因名称 Ager
Gene ID (NCBI) 81722
RRIDAB_3671354
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ63495
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Advanced glycosylation end product-specific receptor (AGER, also known as RAGE) is a member of the immunoglobulin superfamily of cell surface receptors, which interacts with distinct families of ligands, mediating diverse functions in a broad array of cell types including cellular migration, proliferation, survival and apoptosis (PMID: 12645002; 17425919). It senses endogenous stress signals with a broad ligand repertoire including advanced glycation end products, S100 proteins, high-mobility group box 1 protein/HMGB1, amyloid beta/APP oligomers, nucleic acids, phospholipids and glycosaminoglycans (PMID: 19910580; 28627626). It interacts with distinct molecules implicated in homeostasis, development, inflammation, and certain diseases such as diabetes and Alzheimer's disease (PMID: 26253613; 31079281).

实验方案

Product Specific Protocols
WB protocol for AGER/RAGE antibody 83759-4-RRDownload protocol
IF protocol for AGER/RAGE antibody 83759-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols