Sandwich ELISA standard curve of MP00698-3, Rat Cxcl1 Recombinant Matched Antibody Pair - PBS only. 83745-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0948. 83745-2-PBS was HRP conjugated as the detection antibody. Range: 31.3-2000 pg/mL

Rat lung (1 lung, 1-2 mm pieces) were cultured for 3 days in RPMI1640 plus 15% fetal bovine serum and stimulated with 5 μg/mL LPS. An aliquot of cell culture supernatants was removed, The cell culture supernatants was assayed for rat Cxcl1 and measured 465.9 pg/mL; Rat spleen (1/2 spleen; 1-2 mm pieces) were cultured for 3 days in 50 mL DMEM supplemented with 10% fetal bovine serum and stimulated with 5 μg/mL LPS. An aliquot of cell culture supernatants was removed, The cell culture supernatants was assayed for rat Cxcl1 and measured 17,149.5 pg/mL.

Serum of sixteen rats was measured. The rat Cxcl1 concentration of detected samples was determined to be 357.1 pg/mL with a range of 143.6 - 694.0 pg/mL.