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验证数据展示

  • WB analysis of BxPC-3 using 83733-3-RR

    WB analysis of BxPC-3 using 83733-3-RR

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 83733-3-RR (ARID1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 83733-3-RR

    WB analysis using 83733-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83733-3-RR (ARID1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 83733-3-RR

    IHC staining of human colon cancer using 83733-3-RR

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 83733-3-RR (ARID1A antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ARID1A antibody included
  • IHC staining of human stomach cancer using 83733-3-RR

    IHC staining of human stomach cancer using 83733-3-RR

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 83733-3-RR (ARID1A antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ARID1A antibody included
  • IF Staining of HeLa using 83733-3-RR

    IF Staining of HeLa using 83733-3-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ARID1A antibody (83733-3-RR, Clone: 240690F1 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • IF Staining of HeLa using 83733-3-RR

    IF Staining of HeLa using 83733-3-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ARID1A antibody (83733-3-RR, Clone: 240690F1 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 83733-3-RR

    Affinity and Kinetic Characterization of 83733-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83733-3-RR against Human ARID1A were performed. The affinity constant is 0.462 nM.

ARID1A Recombinant monoclonal antibody

ARID1A Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 83733-3-RR

产品说明书

CloneNo. 240690F1

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

BAF250A, BAF250, B120, AT-rich interactive domain-containing protein 1A, ARID domain-containing protein 1A

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inMCF-7 cells, BxPC-3 cells, K-562 cells, HepG2 cells
Positive IHC detected inhuman colon cancer tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy ARID1A Ready-To-Use IHC Kit. ARID1A primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:150-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83733-3-RR targets ARID1A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag30749

Product name: Recombinant human ARID1A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1230-1370 aa of NM_006015

Sequence: KDPYGSMRKAPGSDPFMSSGQGPNGGMGDPYSRAAGPGLGNVAMGPRQHYPYGGPYDRVRTEPGIGPEGNMSTGAPQPNLMPSNPDSGMYSPSRYPPQQQQQQQQRHDSYGNQFSTQGTPSGSPFPSQQTTMYQQQQQNYK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 AT rich interactive domain 1A (SWI-like)
别名 BAF250A, BAF250, B120, AT-rich interactive domain-containing protein 1A, ARID domain-containing protein 1A
计算分子量 242 kDa
观测分子量250-260 kDa
GenBank蛋白编号NM_006015
基因名称 ARID1A
Gene ID (NCBI) 8289
RRIDAB_3671333
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO14497
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ARID1A, also named as BAF250, BAF250A, C1orf4, OSA1 and SMARCF1, is involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). It binds DNA non-specifically. It is also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. ARID1A belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The antibody is specific to ARID1A.

实验方案

Product Specific Protocols
IF protocol for ARID1A antibody 83733-3-RRDownload protocol
IHC protocol for ARID1A antibody 83733-3-RRDownload protocol
WB protocol for ARID1A antibody 83733-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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