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验证数据展示

  • WB analysis using 83722-1-RR (same clone as 83722-1-PBS)

    WB analysis using 83722-1-RR (same clone as 83722-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83722-1-RR (BBS5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83722-1-PBS in a different storage buffer formulation.

  • IF Staining of MDCK using 83722-1-RR (same clone as 83722-1-PBS)

    IF Staining of MDCK using 83722-1-RR (same clone as 83722-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MDCK cells using BBS5 antibody (83722-1-RR, Clone: 240674B5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CoraLite®594 ARL13B antibody (CL594-17711, red). This data was developed using the same antibody clone with 83722-1-PBS in a different storage buffer formulation.

  • WB analysis using 83722-1-RR (same clone as 83722-1-PBS)

    WB analysis using 83722-1-RR (same clone as 83722-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83722-1-RR (BBS5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83722-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00703-1

    Cytometric bead array standard curve of MP00703-1

    Cytometric bead array standard curve of MP00703-1, BBS5 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83722-2-PBS. Detection antibody: 83722-1-PBS. Standard: Ag6153. Range: 2.5-80 ng/mL.

BBS5 Recombinant antibody, PBS Only (Detector)

BBS5 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 83722-1-PBS

产品说明书

CloneNo. 240674B5

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, Cytometric bead array, Indirect ELISA

别名

240674B5, Bardet Biedl syndrome 5, Bardet-Biedl syndrome 5 protein

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

83722-1-PBS targets BBS5 as part of a matched antibody pair:

MP00703-1: 83722-2-PBS capture and 83722-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 BBS5 fusion protein Ag6153 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 Bardet-Biedl syndrome 5
别名 240674B5, Bardet Biedl syndrome 5, Bardet-Biedl syndrome 5 protein
计算分子量 39 kDa
观测分子量39 kDa
GenBank蛋白编号BC044593
基因名称 BBS5
Gene ID (NCBI) 129880
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ8N3I7
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

BBS5 encodes a protein that has been directly linked to Bardet-Biedl syndrome. Bardet-Biedl syndrome (BBS) is an autosomal recessive condition characterised by rod-cone dystrophy, postaxial polydactyly, central obesity, mental retardation, hypogonadism, and renal dysfunction. Other associated clinical findings in BBS patients include diabetes, hypertension and congenital heart defects. BBS expression varies both within and between families and diagnosis is often difficult. Experimentation in non-human eukaryotes suggests that BBS5 is expressed in ciliated cells and that it is required for the formation of cilia. Alternate transcriptional splice variants have been observed but have not been fully characterized.