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验证数据展示

  • IF Staining of HepG2 using 83656-3-RR

    IF Staining of HepG2 using 83656-3-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using TMEM59 antibody (83656-3-RR, Clone: 240457A1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • FC experiment of HepG2 using 83656-3-RR

    FC experiment of HepG2 using 83656-3-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug TMEM59 Recombinant antibody (83656-3-RR, Clone:240457A1) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83656-3-RR

    Affinity and Kinetic Characterization of 83656-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83656-3-RR against Human TMEM59 were performed. The affinity constant is below 1 pM.

TMEM59 Recombinant monoclonal antibody

TMEM59 Uni-rAb® Recombinant Antibody for IF/ICC, FC (Intra), ELISA
Cat No. 83656-3-RR

产品说明书

CloneNo. 240457A1

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra), ELISA

别名

UNQ169/PRO195, Liver membrane-bound protein, HSPC001, C1orf8, 240457A1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy TMEM59 Ready-To-Use IHC Kit. TMEM59 primary antibody included.

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83656-3-RR targets TMEM59 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag1968

Product name: Recombinant human TMEM59 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-324 aa of BC016374

Sequence: MAAPKGSLWVRTQLGLPPLLLLTMALAGGSGTASAEAFDSVLGDTASCHRACQLTYPLHTYPKEEELYACQRGCRLFSICQFVDDGIDLNRTKLECESACTEAYSQSDEQYACHLGCQNQLPFAELRQEQLMSLMPKMHLLFPLTLVRSFWSDMMDSAQSFITSSWTFYLQADDGKIVIFQSKPEIQYAPHLEQEPTNLRESSLSKMSSDLQMRNSQAHRNFLEDGESDGFLRCLSLNSGWILTTTLVLSVMVLLWICCATVATAVEQYVPSEKLSIYGDLEFMNEQKLNRYPASSLVVVRSKTEDHEEAGPLPTKVNLAHSEI

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 transmembrane protein 59
别名 UNQ169/PRO195, Liver membrane-bound protein, HSPC001, C1orf8, 240457A1
计算分子量 36 kDa
GenBank蛋白编号BC016374
基因名称 TMEM59
Gene ID (NCBI) 9528
RRIDAB_3671264
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ9BXS4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TMEM59 is a regulator of autophagy in response to bacterial infection. Overexpression of this protein has been found to promote apoptosis in a glioma cell line. Alternative splicing of TMEM59 results in multiple transcript variants.

实验方案

Product Specific Protocols
FC protocol for TMEM59 antibody 83656-3-RRDownload protocol
IF protocol for TMEM59 antibody 83656-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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