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验证数据展示

  • WB analysis using 83626-5-RR

    WB analysis using 83626-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83626-5-RR (C1orf83 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of U2OS using 83626-5-RR

    IF Staining of U2OS using 83626-5-RR

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using C1orf83 antibody (83626-5-RR, Clone: 240678F1 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 83626-5-RR

    Affinity and Kinetic Characterization of 83626-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83626-5-RR against Human C1orf83 were performed. The affinity constant is below 1 pM.

C1orf83 Recombinant antibody

C1orf83 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 83626-5-RR

产品说明书

CloneNo. 240678F1

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

Transcription elongation factor A N-terminal and central domain-containing protein 2, TCEANC2, RP4 758J24.3, FLJ39169, FLJ32112

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, Jurkat cells, K-562 cells
Positive IF/ICC detected inU2OS cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83626-5-RR targets C1orf83 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 C1orf83 fusion protein Ag15040 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 chromosome 1 open reading frame 83
别名 Transcription elongation factor A N-terminal and central domain-containing protein 2, TCEANC2, RP4 758J24.3, FLJ39169, FLJ32112
计算分子量 208 aa, 23 kDa
观测分子量 24 kDa
GenBank蛋白编号BC035374
基因名称 C1orf83
Gene ID (NCBI) 127428
RRIDAB_3671239
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ96MN5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Chromosome 1 open reading frame 83 (C1orf83) is also called TCEANC2, a little-studied transcription elongation factor. The rs10788972 variant of the TCEANC2 gene in the PARK10 region is closely associated with sporadic PD in the American population, while both rs10788972 and rs12046178 variants are not associated with sporadic PD in the Chinese Han population (PMID: 25663231; PMID: 26432391).

实验方案

Product Specific Protocols
WB protocol for C1orf83 antibody 83626-5-RRDownload protocol
IF protocol for C1orf83 antibody 83626-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols