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验证数据展示

  • WB analysis using 83514-3-RR

    WB analysis using 83514-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83514-3-RR (Integrin beta-5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 83514-3-RR

    IHC staining of human ovary cancer using 83514-3-RR

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83514-3-RR (Integrin beta-5 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human ovary cancer using 83514-3-RR

    IHC staining of human ovary cancer using 83514-3-RR

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83514-3-RR (Integrin beta-5 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • FC experiment of HeLa using 83514-3-RR

    FC experiment of HeLa using 83514-3-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Integrin beta-5 Recombinant antibody (83514-3-RR, Clone:240401G11) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83514-3-RR

    Affinity and Kinetic Characterization of 83514-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83514-3-RR against Human Integrin beta-5 were performed. The affinity constant is 0.955 nM.

Integrin beta-5 Recombinant monoclonal antibody

Integrin beta-5 Uni-rAb® Recombinant Antibody for WB, IHC, FC (Intra), ELISA
Cat No. 83514-3-RR

产品说明书

CloneNo. 240401G11

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, FC (Intra), ELISA

别名

ITGB5, 240401G11, Integrin beta 5, integrin, beta 5

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA549 cells, HT-29 cells, NIH-H1299 cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83514-3-RR targets Integrin beta-5 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag29783

Product name: Recombinant human Integrin beta-5 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 24-126 aa of BC006541

Sequence: GLNICTSGSATSCEECLLIHPKCAWCSKEDFGSPRSITSRCDLRANLVKNGCGGEIESPASSFHVLRSLPLSSKGSGSAGWDVIQMTPQEIAVNLRPGDKTTF

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 integrin, beta 5
别名 ITGB5, 240401G11, Integrin beta 5, integrin, beta 5
计算分子量 88 kDa
观测分子量88-100 kDa
GenBank蛋白编号BC006541
基因名称 Integrin beta 5
Gene ID (NCBI) 3693
RRIDAB_3671139
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP18084
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ITGB5, also known as Integrin beta-5. It is expected to be located in the plasma membrane and mitochondria, which is i ubiquitinated in placenta and gall bladder. ITGB5 is a member of integrins and was proved to be associated with pathological processes in several tumors (PMID: 34966851). It is reported that TGB5 is highly expressed in hepatocellular carcinoma (HCC), and miR-185 regulates the expression of β- catenin through the ITGB5-dependent manner and affects the proliferation and migration of HCC cells (PMID: 35223869). The molecular weight of ITGB5 is 88 kDa, and the glycosylation modification also exists.

实验方案

Product Specific Protocols
WB protocol for Integrin beta-5 antibody 83514-3-RRDownload protocol
IHC protocol for Integrin beta-5 antibody 83514-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols