验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, MDA-MB-231 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
83499-3-RR targets UPK3A in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | uroplakin 3A |
| 别名 | Uroplakin III, uroplakin 3A, UPK3, UPIIIA, UPIII |
| 计算分子量 | 31 kDa |
| 观测分子量 | 47 kDa |
| GenBank蛋白编号 | NM_006953 |
| 基因名称 | UPK3A |
| Gene ID (NCBI) | 7380 |
| RRID | AB_3671125 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purfication |
| UNIPROT ID | O75631 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Uroplakins are a group of urothelial differentiation-related membrane proteins. They are components of the asymmetric unit membrane (AUM), which forms the apical plaques of mammalian urothelium and is believed to play a role in strengthening the urothelial apical surface thus preventing the cells from rupturing during bladder distention (PMID: 8175808). Uroplakin III (UPK3A) is a promising new urine marker for bladder cancer detection (PMID: 20346489). Loss of UPK3A expression is associated with clinicopathological features of invasive bladder cancer and poor prognosis in upper tract urothelial carcinoma (PMID: 18313120; PMID: 16686732). UPK3A has a calculated molecular weight of 30.7 kDa, with an apparent molecular weight of 47 kDa due to three potential N-glycosylation sites.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for UPK3A antibody 83499-3-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |