验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, K-562 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
83462-1-RR targets RNF133 in WB, ELISA applications and shows reactivity with Human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | Human |
| 免疫原 |
CatNo: Ag14537 Product name: Recombinant human RNF133 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 208-376 aa of BC022038 Sequence: YHIHRLCLARIQNRRWQRLTTDLQNTFGQLQLRVVKEGDEEINPNGDSCVICFERYKPNDIVRILTCKHFFHKNCIDPWILPHGTCPICKCDILKVLGIQVVVENGTEPLQVLMSNELPETLSPSEEETNNEVSPAGTSDKVIHVEENPTSQNNDIQPHSVVEDVHPSP 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | ring finger protein 133 |
| 别名 | RING-type E3 ubiquitin transferase RNF133, RING finger protein 133, EC:2.3.2.27, E3 ubiquitin-protein ligase RNF133, 240397C12 |
| 计算分子量 | 376 aa, 42 kDa |
| 观测分子量 | 47 kDa |
| GenBank蛋白编号 | BC022038 |
| 基因名称 | RNF133 |
| Gene ID (NCBI) | 168433 |
| RRID | AB_3671093 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q8WVZ7 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RNF133 (ring finger protein 133) contains a RING finger domain, a motif present in a variety of functionally distinct proteins and known to be involved in protein-protein and protein-DNA interactions.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RNF133 antibody 83462-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
