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验证数据展示

  • IF Staining of RAW 264.7 using 83413-5-RR (same clone as 83413-5-PBS)

    IF Staining of RAW 264.7 using 83413-5-RR (same clone as 83413-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using Osteopontin antibody (83413-5-RR, Clone: 240250H3 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83413-5-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP00400-2

    Sandwich ELISA standard curve of MP00400-2

    Sandwich ELISA standard curve of MP00400-2, mouse/rat Osteopontin Recombinant Matched Antibody Pair - PBS only. 83413-5-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0317. 83413-4-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL.

  • Sample test of MP00400-2

    Sample test of MP00400-2

    Serum of seven rats was measured. The Osteopontin concentration of detected samples was determined to be 12.8 ng/mL with a range of 1.1 - 43.0 ng/mL.

  • Sample test of MP00400-2

    Sample test of MP00400-2

    J774A.1 mouse reticulum cell sarcoma macrophage cells were cultured in DMEM supplemented with 10% fetal bovine serum, 2.5 mM L-glutamine, 100 U/mL penicillin and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernatant was removed, assayed for mouse/rat Osteopontin and measured 20.0 ng/mL.

  • Sample test of MP00400-2

    Sample test of MP00400-2

    Serum of sixteen mice was measured. The Osteopontin concentration of detected samples was determined to be 32.0 ng/mL with a range of 6.2 - 53.0 ng/mL.

  • Cytometric bead array standard curve of MP00400-2

    Cytometric bead array standard curve of MP00400-2

    Cytometric bead array standard curve of MP00400-2, MOUSE Osteopontin Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83413-5-PBS. Detection antibody: 83413-4-PBS. Standard: Eg0317. Range: 0.313-40 ng/mL.

  • Cytometric bead array standard curve of MP00400-3

    Cytometric bead array standard curve of MP00400-3

    Cytometric bead array standard curve of MP00400-3, MOUSE Osteopontin Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83413-2-PBS. Detection antibody: 83413-5-PBS. Standard: Eg0317. Range: 0.625-40 ng/mL.

  • Affinity and Kinetic Characterization of 83413-5-RR

    Affinity and Kinetic Characterization of 83413-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83413-5-RR against Mouse Osteopontin were performed. The affinity constant is 4.36 nM.

Mouse Osteopontin Recombinant antibody, PBS Only (Capture/Detector)

Osteopontin Uni-rAbTM Recombinant Antibody for IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 83413-5-PBS

产品说明书

CloneNo. 240250H3

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

别名

Spp1, Opn, 240250H3

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

83413-5-PBS targets Osteopontin as part of a matched antibody pair:

MP00400-2: 83413-5-PBS capture and 83413-4-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

MP00400-3: 83413-2-PBS capture and 83413-5-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 mouse
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 secreted phosphoprotein 1
别名 Spp1, Opn, 240250H3
计算分子量32kd
GenBank蛋白编号NM_001204233.1
基因名称 Osteopontin
Gene ID (NCBI) 20750
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ547B5
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Osteopontin (OPN) is also named as 2AR, minopontin, bone sialoprotein 1, Spp1, Eta-1 and Op, and belongs to the osteopontin family. Osteopontin is major non-collagenous bone protein that binds tightly to hydroxyapatite. It is a highly phosphorylated glycophosphoprotein having acidic characteristics and rich in aspartic acid (PMID: 30003880). And it acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity (PMID:2351930, PMID:10657301). OPN, a multifunctional protein, has important functions on cardiovascular diseases, cancer, diabetes and kidney stone diseases and in the process of inflammation, biomineralization, cell viability and wound healing (PMID: 30003880). Osteopontin (OPN) is a pleiotropic glycoprotein expressed in various cell types in animals and in humans, including bone, immune, smooth muscle, epithelial and endothelial cells. Moreover, OPN is found in kidneys (in the thick ascending limbs of the loop of Henle and in distal nephrons) and urine (PMID:30456594). Osteopontin (OPN) is an acidic 60-70 kDa glycoprotein that is cleaved by proteases to yield 45-kDa and 24-kDa fragments (PMID: 10491621, PMID: 1995615)