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验证数据展示

  • WB analysis using 83410-3-RR

    WB analysis using 83410-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83410-3-RR (TRNP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • FC experiment of HeLa using 83410-3-RR

    FC experiment of HeLa using 83410-3-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug TRNP1 Recombinant antibody (83410-3-RR, Clone:240331C6) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 83410-3-RR

    Affinity and Kinetic Characterization of 83410-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83410-3-RR against Human TRNP1 were performed. The affinity constant is 72.8 pM.

TRNP1 Recombinant antibody

TRNP1 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 83410-3-RR

产品说明书

CloneNo. 240331C6

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, FC (Intra), ELISA

别名

TRNP, TNRP, TMF-regulated nuclear protein 1, C1orf225, 240331C6

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, MCF-7 cells, Jurkat cells, SH-SY5Y cells, rat heart tissue
Positive FC (Intra) detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83410-3-RR targets TRNP1 in WB, FC (Intra), ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human, rat
免疫原 TRNP1 fusion protein Ag22504 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 TMF1-regulated nuclear protein 1
别名 TRNP, TNRP, TMF-regulated nuclear protein 1, C1orf225, 240331C6
计算分子量 227 aa, 23 kDa
观测分子量 23-25 kDa
GenBank蛋白编号BC069216
基因名称 TRNP1
Gene ID (NCBI) 388610
RRIDAB_3671057
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ6NT89
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TRNP1 (TMF-regulated nuclear protein 1), also called C1orf225, is a DNA-binding factor that regulates the expression of a subset of genes and plays a key role in tangential, radial, and lateral expansion of the brain neocortex (PMID: 27264089; 23622239; 36947129). It has been shown to exert potent roles in neural development affecting neural stem cell self-renewal and brain folding (PMID: 32627867). It may also control the proliferation rate of cells by regulating their progression through key cell-cycle transition points.

实验方案

Product Specific Protocols
WB protocol for TRNP1 antibody 83410-3-RRDownload protocol
FC protocol for TRNP1 antibody 83410-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols