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验证数据展示

  • WB analysis using 83311-4-RR

    WB analysis using 83311-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83311-4-RR (MUC1/CA15-3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach cancer using 83311-4-RR

    IHC staining of human stomach cancer using 83311-4-RR

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 83311-4-RR (MUC1/CA15-3 C-terminal antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer(pH9).

    Ready-to-use IHC kits available with MUC1 antibody included
  • IHC staining of human tonsillitis using 83311-4-RR

    IHC staining of human tonsillitis using 83311-4-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 83311-4-RR (MUC1/CA15-3 C-terminal antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MUC1 antibody included
  • IHC staining of human rectal cancer using 83311-4-RR

    IHC staining of human rectal cancer using 83311-4-RR

    Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 83311-4-RR (MUC1/CA15-3 C-terminal antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MUC1 antibody included
  • IHC staining of human ovary cancer using 83311-4-RR

    IHC staining of human ovary cancer using 83311-4-RR

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83311-4-RR (MUC1/CA15-3 C-terminal antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MUC1 antibody included
  • Affinity and Kinetic Characterization of 83311-4-RR

    Affinity and Kinetic Characterization of 83311-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83311-4-RR against Human CA15-3/MUC1 were performed. The affinity constant is 63.7 pM.

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MUC1/CA15-3 C-terminal Recombinant monoclonal antibody

MUC1/CA15-3 C-terminal Uni-rAb® Recombinant Antibody for WB, IHC, ELISA
Cat No. 83311-4-RR

产品说明书

CloneNo. 240190G10

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

CA15-3, CA153, MUC1, CA15 3, CA 15-3

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inBxPC-3 cells, mouse lung tissue
Positive IHC detected inhuman stomach cancer tissue, human ovary cancer tissue, human rectal cancer tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy MUC1 Ready-To-Use IHC Kit. MUC1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83311-4-RR targets MUC1/CA15-3 C-terminal in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag20366

Product name: Recombinant human CA15-3,MUC1 protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 1055-1255 aa of BC120975

Sequence: NSSLEDPSTDYYQELQRDISEMFLQIYKQGGFLGLSNIKFRPGSVVVQLTLAFREGTINVHDVETQFNQYKTEAASRYNLTISDVSVSDVPFPFSAQSGAGVPGWGIALLVLVCVLVALAIVYLIALAVCQCRRKNYGQLDIFPARDTYHPMSEYPTYHTHGRYVPPSSTDRSPYEKVSAGNGGSSLSYTNPAVAATSANL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 mucin 1, cell surface associated
别名 CA15-3, CA153, MUC1, CA15 3, CA 15-3
计算分子量 1264 aa, 123 kDa
观测分子量 23-30 kDa
GenBank蛋白编号BC120975
基因名称 MUC1/CA15-3
Gene ID (NCBI) 4582
RRIDAB_3670978
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDP15941
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MUC1 is a type I transmembrane glycoprotein expressed by various epithelial cells of the female reproductive tract, lung, breast, kidney, stomach, and pancreas. MUC1 is transcribed as a large precursor gene product, and upon translation, is cleaved in the endoplasmic reticulum, yielding two subunits: the large extracellular N-terminal subunit (MUC1-N, about 120-200 kDa) and the small cytoplasmic C-terminal subunit (MUC1-C, about 23-30 kDa). Among the known mucins, MUC1 is best studied and plays crucial roles in regulating many cellular properties, including cell proliferation, apoptosis, adhesion, and invasion. MUC1 is overexpressed in a wide range of human epithelial malignancies. This antibody was raised against the C-terminal region of human MUC1, thus it recognizes the 23-30 kDa cytoplasmic MUC1.

实验方案

Product Specific Protocols
WB protocol for MUC1/CA15-3 C-terminal antibody 83311-4-RRDownload protocol
IHC protocol for MUC1/CA15-3 C-terminal antibody 83311-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols