验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, MCF-7 cells, NIH/3T3 cells, mouse testis tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
83290-3-RR targets GAPDHS in WB, ELISA applications and shows reactivity with Human, mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | Human, mouse |
| 免疫原 |
CatNo: Ag4988 Product name: Recombinant human GAPDHS protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 58-408 aa of BC036373 Sequence: LPPHPATPPPKMVSVARELTVGINGFGRIGRLVLRACMEKGVKVVAVNDPFIDPEYMVYMFKYDSTHGRYKGSVEFRNGQLVVDNHEISVYQCKEPKQIPWRAVGSPYVVESTGVYLSIQAASDHISAGAQRVVISAPSPDAPMFVMGVNENDYNPGSMNIVSNASCTTNCLAPLAKVIHERFGIVEGLMTTVHSYTATQKTVDGPSRKAWRDGRGAHQNIIPASTGAAKAVTKVIPELKGKLTGMAFRVPTPDVSVVDLTCRLAQPAPYSAIKEAVKAAAKGPMAGILAYTEDEVVSTDFLGDTHSSIFDAKAGIALNDNFVKLISWYDNEYGYSHRVVDLLRYMFSRDK 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | glyceraldehyde-3-phosphate dehydrogenase, spermatogenic |
| 别名 | GAPD2, GAPDH 2, GAPDH2, GAPDHS, GAPDS, HSD 35 |
| 计算分子量 | 45 kDa |
| 观测分子量 | 60-65 kDa |
| GenBank蛋白编号 | BC036373 |
| 基因名称 | GAPDHS |
| Gene ID (NCBI) | 26330 |
| RRID | AB_3670957 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purfication |
| UNIPROT ID | O14556 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GAPDHS(Glyceraldehyde-3-phosphate dehydrogenase, testis-specific) is also named as GAPD2, GAPDH2, GAPDS and belongs to the glyceraldehyde-3-phosphate dehydrogenase family. It may play an important role in regulating the switch between different pathways for energy production during spermiogenesis and in the spermatozoon. GAPDHS is a promising contraceptive target because it is specific to male germ cells, essential for sperm motility and male fertility, and well suited to pharmacological inhibition(PMID:20828617).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GAPDHS antibody 83290-3-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
