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验证数据展示

  • WB analysis using 83187-3-RR

    WB analysis using 83187-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83187-3-RR (SMC6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of human testis using 83187-3-RR

    WB analysis of human testis using 83187-3-RR

    human testis tissue were subjected to SDS PAGE followed by western blot with 83187-3-RR (SMC6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse testis using 83187-3-RR

    IF Staining of mouse testis using 83187-3-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using SMC6 antibody (83187-3-RR, Clone: 240015F4 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • FC experiment of A431 using 83187-3-RR

    FC experiment of A431 using 83187-3-RR

    1x10^6 A431 cells were intracellularly stained with 0.25 ug SMC6 Recombinant antibody (83187-3-RR, Clone:240015F4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of mouse testis using 83187-3-RR

    IF Staining of mouse testis using 83187-3-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using SMC6 antibody (83187-3-RR, Clone: 240015F4 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 83187-3-RR

    Affinity and Kinetic Characterization of 83187-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83187-3-RR against Human SMC6 were performed. The affinity constant is 0.428 nM.

SMC6 Recombinant antibody

SMC6 Recombinant Antibody for WB, IF-P, FC (Intra), ELISA
Cat No. 83187-3-RR

产品说明书

CloneNo. 240015F4

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF-P, FC (Intra), ELISA

别名

SMC6L1, SMC-6, SMC protein 6, SMC 6, hSMC6

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, human testis tissue, NIH/3T3 cells, HEK-293 cells
Positive IF-P detected inmouse testis tissue
Positive FC (Intra) detected inA431 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83187-3-RR targets SMC6 in WB, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF-P, FC (Intra), ELISA Application Description
经测试反应性 human, mouse
免疫原 SMC6 fusion protein Ag6898 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 structural maintenance of chromosomes 6
别名 SMC6L1, SMC-6, SMC protein 6, SMC 6, hSMC6
计算分子量 126 kDa
观测分子量 130 kDa
GenBank蛋白编号BC039828
基因名称 SMC6
Gene ID (NCBI) 79677
RRIDAB_3670876
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ96SB8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SMC6 (Structural maintenance of chromosomes protein 6) is core component of the SMC5-SMC6 complex that is involved in DNA double-strand breaks by homologous recombination. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks. The complex is required for telomere maintenance via recombination in ALT (alternative lengthening of telomeres) cell lines and mediates sumoylation of shelterin complex (telosome) components which is proposed to lead to shelterin complex disassembly in ALT-associated PML bodies (APBs) (PMID: 26983541).

实验方案

Product Specific Protocols
WB protocol for SMC6 antibody 83187-3-RRDownload protocol
IF protocol for SMC6 antibody 83187-3-RRDownload protocol
FC protocol for SMC6 antibody 83187-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols