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验证数据展示

  • WB analysis using 83042-3-RR

    WB analysis using 83042-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83042-3-RR (MTA3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • FC experiment of U-251 using 83042-3-RR

    FC experiment of U-251 using 83042-3-RR

    1x10^6 U-251 cells were intracellularly stained with 0.25 ug MTA3 Recombinant antibody (83042-3-RR, Clone:230350D12) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • IF Staining of U-251 using 83042-3-RR

    IF Staining of U-251 using 83042-3-RR

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using MTA3 antibody (83042-3-RR, Clone: 230350D12 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

  • WB analysis of MCF-7 using 83042-3-RR

    WB analysis of MCF-7 using 83042-3-RR

    WB result of MTA3 antibody (83042-3-RR; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MTA3 transfected MCF-7 cells.

  • Affinity and Kinetic Characterization of 83042-3-RR

    Affinity and Kinetic Characterization of 83042-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83042-3-RR against Human MTA3 were performed. The affinity constant is below 1 pM.

  • Featured Product
  • KD/KO验证

MTA3 Recombinant monoclonal antibody

MTA3 Uni-rAb® Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 83042-3-RR

产品说明书

CloneNo. 230350D12

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, FC (Intra), ELISA

别名

230350D12, Metastasis-associated protein MTA3, MTA 3, mta1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 750
规格价格库存

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经过测试的应用

Positive WB detected inJurkat cells, MCF-7 cells, A375 cells, NIH/3T3 cells, C2C12 cells, Raji cells
Positive IF/ICC detected inU-251 cells
Positive FC (Intra) detected inU-251 cells
Planning an IHC experiment? We recommend our IHCeasy MTA3 Ready-To-Use IHC Kit. MTA3 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83042-3-RR targets MTA3 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag6400

Product name: Recombinant human MTA3 protein

Source: e coli.-derived, PKG

Tag: GST

Domain: 1-343 aa of BC053631

Sequence: MAANMYRVGDYVYFENSSSNPYLIRRIEELNKTASGNVEAKVVCFYRRRDISNTLIMLADKHAKEIEEESETTVEADLTDKQKHQLKHRELFLSRQYESLPATHIRGKCSVALLNETESVLSYLDKEDTFFYSLVYDPSLKTLLADKGEIRVGPRYQADIPEMLLEGESDEREQSKLEVKVWDPNSPLTDRQIDQFLVVARAVGTFARALDCSSSVRQPSLHMSAAAASRDITLFHAMDTLYRHSYDLSSAISVLVPLGGPVLCRDEMEEWSASEASLFEEALEKYGKDFNDIRQDFLPWKSLTSIIEYYYMWKTTDRYVQQKRLKAAEAESKLKQVYIPTYS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 metastasis associated 1 family, member 3
别名 230350D12, Metastasis-associated protein MTA3, MTA 3, mta1
计算分子量 68 kDa
观测分子量62 kDa, 68 kDa
GenBank蛋白编号BC053631
基因名称 MTA3
Gene ID (NCBI) 57504
RRIDAB_3670772
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ9BTC8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The metastasis-associated (MTA) family contains three members, MTA1, MTA2 and MTA3 (PMID:12167865).Metastasis-associated protein 3 (MTA3) is a cell-type specific component of the Mi-2-NURD transcriptional co-repressor complex. It has a role in maintenance of the normal epithelial architecture through the repression of SNAI1 transcription in a histone deacetylase-dependent manner, and thus the regulation of E-cadherin levels.

实验方案

Product Specific Protocols
IF protocol for MTA3 antibody 83042-3-RRDownload protocol
WB protocol for MTA3 antibody 83042-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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