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验证数据展示

  • WB analysis using 82993-6-RR

    WB analysis using 82993-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82993-6-RR (Endoglin/CD105 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 82993-6-RR

    IHC staining of human ovary cancer using 82993-6-RR

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 82993-6-RR (Endoglin/CD105 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Endoglin/CD105 antibody included
  • IHC staining of human kidney using 82993-6-RR

    IHC staining of human kidney using 82993-6-RR

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 82993-6-RR (Endoglin/CD105 antibody) at dilution of 1:2000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • IHC staining of human liver using 82993-6-RR

    IHC staining of human liver using 82993-6-RR

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 82993-6-RR (Endoglin/CD105 antibody) at dilution of 1:2000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • IHC staining of human appendicitis using 82993-6-RR

    IHC staining of human appendicitis using 82993-6-RR

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 82993-6-RR (Endoglin/CD105 antibody) at dilution of 1:2000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • IHC staining of human ovary cancer using 82993-6-RR

    IHC staining of human ovary cancer using 82993-6-RR

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 82993-6-RR (Endoglin/CD105 antibody) at dilution of 1:2000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • IHC staining of human tonsillitis using 82993-6-RR

    IHC staining of human tonsillitis using 82993-6-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 82993-6-RR (Endoglin/CD105 antibody) at dilution of 1:2000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • IHC staining of human placenta using 82993-6-RR

    IHC staining of human placenta using 82993-6-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 82993-6-RR (Endoglin/CD105 antibody) at dilution of 1:2000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • IHC staining of human colorectal cancer using 82993-6-RR

    IHC staining of human colorectal cancer using 82993-6-RR

    Immunohistochemical analysis of paraffin-embedded human colorectal cancer tissue slide using 82993-6-RR (Endoglin/CD105 antibody) at dilution of 1:2000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • IHC staining of human pancreas cancer using 82993-6-RR

    IHC staining of human pancreas cancer using 82993-6-RR

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 82993-6-RR (Endoglin/CD105 antibody) at dilution of 1:2000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • IHC staining of human ovary cancer using 82993-6-RR

    IHC staining of human ovary cancer using 82993-6-RR

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 82993-6-RR (Endoglin/CD105 antibody) at dilution of 1:2000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Endoglin/CD105 antibody included
  • IHC staining of human colorectal cancer using 82993-6-RR

    IHC staining of human colorectal cancer using 82993-6-RR

    Immunohistochemical analysis of paraffin-embedded human colorectal cancer tissue slide using 82993-6-RR (Endoglin/CD105 antibody) at dilution of 1:2000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Endoglin/CD105 antibody included
  • IHC staining of human pancreas cancer using 82993-6-RR

    IHC staining of human pancreas cancer using 82993-6-RR

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 82993-6-RR (Endoglin/CD105 antibody) at dilution of 1:2000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Endoglin/CD105 antibody included
  • IHC staining of human liver cancer using 82993-6-RR

    IHC staining of human liver cancer using 82993-6-RR

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 82993-6-RR (Endoglin/CD105 antibody) at dilution of 1:2000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Endoglin/CD105 antibody included
  • IHC staining of human tonsillitis using 82993-6-RR

    IHC staining of human tonsillitis using 82993-6-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 82993-6-RR (Endoglin/CD105 antibody) at dilution of 1:2000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Endoglin/CD105 antibody included
  • IHC staining of human tonsillitis using 82993-6-RR

    IHC staining of human tonsillitis using 82993-6-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 82993-6-RR (Endoglin/CD105 antibody) at dilution of 1:2000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Endoglin/CD105 antibody included
  • IHC staining of human liver using 82993-6-RR

    IHC staining of human liver using 82993-6-RR

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 82993-6-RR (Endoglin/CD105 antibody) at dilution of 1:2000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Endoglin/CD105 antibody included
  • IHC staining of human kidney using 82993-6-RR

    IHC staining of human kidney using 82993-6-RR

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 82993-6-RR (Endoglin/CD105 antibody) at dilution of 1:2000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Endoglin/CD105 antibody included
  • IF Staining of human tonsillitis using 82993-6-RR

    IF Staining of human tonsillitis using 82993-6-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using Endoglin/CD105 antibody (82993-6-RR, Clone: 241196G9 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), Endoglin/CD105 antibody (67075-1-Ig, Clone: 2D5E8, red). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of human tonsillitis using 82993-6-RR

    IF Staining of human tonsillitis using 82993-6-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using Endoglin/CD105 antibody (82993-6-RR, Clone: 241196G9 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), Endoglin/CD105 antibody (67075-1-Ig, Clone: 2D5E8, red). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Endoglin/CD105 Recombinant monoclonal antibody

Endoglin/CD105 Uni-rAb® Recombinant Antibody for WB, IHC, IF-P, ELISA
Cat No. 82993-6-RR

产品说明书

CloneNo. 241196G9

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF-P, ELISA

别名

CD105, 241196G9, END, Endoglin, ENG

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, HUVEC cells, JAR cells
Positive IHC detected inhuman ovary cancer tissue, human liver tissue, human liver cancer tissue, human tonsillitis tissue, human pancreas cancer tissue, human colorectal cancer tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman tonsillitis tissue
Planning an IHC experiment? We recommend our IHCeasy Endoglin/CD105 Ready-To-Use IHC Kit. Endoglin/CD105 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82993-6-RR targets Endoglin/CD105 in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Eg0587

Product name: Recombinant Human Endoglin/CD105 protein (His Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: C-6*HIS

Domain: 26-586 aa of BC014271

Sequence: ETVHCDLQPVGPERGEVTYTTSQVSKGCVAQAPNAILEVHVLFLEFPTGPSQLELTLQASKQNGTWPREVLLVLSVNSSVFLHLQALGIPLHLAYNSSLVTFQEPPGVNTTELPSFPKTQILEWAAERGPITSAAELNDPQSILLRLGQAQGSLSFCMLEASQDMGRTLEWRPRTPALVRGCHLEGVAGHKEAHILRVLPGHSAGPRTVTVKVELSCAPGDLDAVLILQGPPYVSWLIDANHNMQIWTTGEYSFKIFPEKNIRGFKLPDTPQGLLGEARMLNASIVASFVELPLASIVSLHASSCGGRLQTSPAPIQTTPPKDTCSPELLMSLIQTKCADDAMTLVLKKELVAHLKCTITGLTFWDPSCEAEDRGDKFVLRSAYSSCGMQVSASMISNEAVVNILSSSSPQRKKVHCLNMDSLSFQLGLYLSPHFLQASNTIEPGQQSFVQVRVSPSVSEFLLQLDSCHLDLGPEGGTVELIQGRAAKGNCVSLLSPSPEGDPRFSFLLHFYTVPIPKTGTLSCTVALRPKTGSQDQEVHRTVFMRLNIISPDLSGCTSKG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 endoglin
别名 CD105, 241196G9, END, Endoglin, ENG
计算分子量 71 kDa
观测分子量75-90 kDa
GenBank蛋白编号BC014271
基因名称 CD105
Gene ID (NCBI) 2022
RRIDAB_3670742
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP17813
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Endoglin (ENG, CD105) is a homodimeric cell membrane glycoprotein of 180 kDa, composed of disulphide-linked subunits of 90-95 kDa. Endoglin is a proliferation-associated and hypoxia-inducible protein mainly expressed on vascular endothelial cells. It acts as an accessory receptor for transforming growth factor beta (TFG-β) and is involved in vascular development and remodelling. The important role of Endoglin in angiogenesis and in tumor progression makes it an ideal target for antiangiogenic therapy and a good marker for tumor prognosis. (PMID: 1326540; 14528280; 21737653; 12773481)

实验方案

Product Specific Protocols
IF protocol for Endoglin/CD105 antibody 82993-6-RRDownload protocol
IHC protocol for Endoglin/CD105 antibody 82993-6-RRDownload protocol
WB protocol for Endoglin/CD105 antibody 82993-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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