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验证数据展示

  • Dot Blot experiment of peptide using 82823-2-RR

    Dot Blot experiment of peptide using 82823-2-RR

    Dot blot analysis was used to confirm the specificity of Acetyl-Histone H2A (Lys9) antibody. Acetylated peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (μg/mL) spotted is indicated next to each row. Column 1: H2AK9ac. Column 2: Unmodified H2AK9. Column 3: H2AK5ac. Column 4: Unmodified H2AK5. Column 5: H2AK13Ac. Column 6: Unmodified H2AK13. Column 7: H2AK15ac. Column 8:Unmodified H2AK15. Column 9: Blank(PBS).

  • ChIP experiment of HeLa using 82823-2-RR

    ChIP experiment of HeLa using 82823-2-RR

    Chromatin was prepared from HeLa cells. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 15 µg of cross-linked chromatin, 5 µg of Acetyl-Histone H2A (Lys9) (82823-2-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR.

  • WB analysis using 82823-2-RR

    WB analysis using 82823-2-RR

    Trichostain A treated and untreated HeLa cell lysates were subjected to SDS PAGE followed by western blot with 82823-2-RR (Acetyl-Histone H2A (Lys9) antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Alpha Tubulin Monoclonal antibody (66031-1-Ig) as loading control.

Acetyl-Histone H2A (Lys9) Recombinant monoclonal antibody

Acetyl-Histone H2A (Lys9) Uni-rAb® Recombinant Antibody for WB, Dot Blot, ELISA, ChIP-qPCR
Cat No. 82823-2-RR

产品说明书

CloneNo. 1O17

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, Dot Blot, ELISA, ChIP-qPCR

别名

Ac Histone H2A, Ac Histone H2A (Lys9), Ac Histone H2A Lys9, Ac Histone H2A-Lys9, Acetyl Histone

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, Trichostain A treated HeLa cells
Positive Dot Blot detected inpeptide
Positive ChIP-qPCR detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy Histone H2A Ready-To-Use IHC Kit. Histone H2A primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:5000
DOT BLOTDOT BLOT : 1:10-1:100
CHIP-QPCRCHIP-QPCR : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82823-2-RR targets Acetyl-Histone H2A (Lys9) in WB, Dot Blot, ELISA, ChIP-qPCR applications and shows reactivity with human samples.

经测试应用 WB, Dot Blot, ELISA, ChIP-qPCR Application Description
经测试反应性 human
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 histone cluster 1, H2aa
别名 Ac Histone H2A, Ac Histone H2A (Lys9), Ac Histone H2A Lys9, Ac Histone H2A-Lys9, Acetyl Histone
观测分子量16 kDa
GenBank蛋白编号BC062211
基因名称 HIST1H2AA
Gene ID (NCBI) 221613
RRIDAB_3670564
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ96QV6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for Acetyl-Histone H2A (Lys9) antibody 82823-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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