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验证数据展示

  • IF Staining of HeLa using 82785-1-RR

    IF Staining of HeLa using 82785-1-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CPEB1 antibody (82785-1-RR, Clone: 6I9 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR004).

  • Affinity and Kinetic Characterization of 82785-1-RR

    Affinity and Kinetic Characterization of 82785-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82785-1-RR against Human CPEB1 were performed. The affinity constant is below 1 pM.

CPEB1 Recombinant monoclonal antibody

CPEB1 Recombinant Antibody for IF/ICC, ELISA
Cat No. 82785-1-RR

产品说明书

CloneNo. 6I9

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, ELISA

别名

CPE-binding protein 1, CPEB, CPE BP1, CPE binding protein 1, CEBP

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82785-1-RR targets CPEB1 in IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag3994

Product name: Recombinant human CPEB1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 188-486 aa of BC035348

Sequence: WDLLEAPKDPFSIEREARLHRQAAAVNEATCTWSGQLPPRNYKNPIYSCKVFLGGVPWDITEAGLVNTFRVFGSLSVEWPGKDGKHPRCPPKGYVYLVFELEKSVRSLLQACSHDPLSPDGLSEYYFKMSSRRMRCKEVQVIPWVLADSNFVRSPSQRLDPSRTVFVGALHGMLNAEALAAILNDLFGGVVYAGIDTDKHKYPIGSGRVTFNNQRSYLKAVSAAFVEIKTTKFTKKVQIDPYLEDSLCHICSSQPGPFFCRDQVCFKYFCRSCWHWRHSMEGLRHHSPLMRNQKNRDSS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 cytoplasmic polyadenylation element binding protein 1
别名 CPE-binding protein 1, CPEB, CPE BP1, CPE binding protein 1, CEBP
计算分子量 486 aa, 54 kDa
观测分子量 63 kDa
GenBank蛋白编号BC035348
基因名称 CPEB1
Gene ID (NCBI) 64506
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9BZB8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CPEB1, also named as CEBP, CPEB and CPE-BP1, contains two RRM (RNA recognition motif) domains and belongs to the RRM CPEB family. It is a sequence-specific RNA-binding protein that regulates mRNA cytoplasmic polyadenylation and translation initiation during oocyte maturation, early development and at postsynapse sites of neurons. CPEB1 regulated translation is a key process involved in INS signaling.

实验方案

Product Specific Protocols
IF protocol for CPEB1 antibody 82785-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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