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验证数据展示

  • WB analysis using 82776-8-RR

    WB analysis using 82776-8-RR

    Non-treated and IGF-1 treated MCF-7 cells were subjected to SDS PAGE followed by western blot with 82776-8-RR (Phospho-ULK1 (Ser758) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was tripped and re-blotted with Alpha Tubulin antibody (66031-1-Ig) as loading control.

  • WB analysis using 82776-8-RR

    WB analysis using 82776-8-RR

    Non-treated, phosphatase inhibitor treated and λ phosphatase treated PC-3 cells were subjected to SDS PAGE followed by western blot with 82776-8-RR (Phospho-ULK1 (Ser758) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was tripped and re-blotted with Vinculin antibody (66305-1-Ig) as loading control.

Phospho-ULK1 (Ser758) Recombinant monoclonal antibody

Phospho-ULK1 (Ser758) Uni-rAb® Recombinant Antibody for WB, ELISA
Cat No. 82776-8-RR

产品说明书

CloneNo. 240770A7

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

P ULK1 (Ser758), Phos ULK1 (Ser758), Phospho-ULK1 (Ser757), Phos-ULK1 (Ser758), P-ULK1 (Ser758)

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inIGF-1 treated MCF-7 cells, PC-3 cells, λ phosphatase treated PC-3 cells

This antibody is equivalent to Ser757 in mice.

Planning an IHC experiment? We recommend our IHCeasy ULK1 Ready-To-Use IHC Kit. ULK1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82776-8-RR targets Phospho-ULK1 (Ser758) in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 unc-51-like kinase 1 (C. elegans)
别名 P ULK1 (Ser758), Phos ULK1 (Ser758), Phospho-ULK1 (Ser757), Phos-ULK1 (Ser758), P-ULK1 (Ser758)
观测分子量 140 kDa
GenBank蛋白编号NM_003565
基因名称 ULK1
Gene ID (NCBI) 8408
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDO75385
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Unc-51-like-kinase 1 (ULK1) is a target of both the mechanistic target of rapamycin (mTOR) and AMP activated protein kinase (AMPK), whose role is to facilitate the initiation of autophagy in response to starvation. ULK1 is phosphorylated on serine 638 and 758 sites by mTOR in nutrient-rich conditions, inhibiting ULK1 activation by disrupting its binding to AMPK. Upon glucose starvation, dissociation of mTOR from ULK1 and phosphorylation by AMPK leads to the activation of ULK1 activity. The S556 site of ULK1 is one of the major AMPK-dependent phosphorylation sites. (PMID: 30517873, PMID: 21258367).This antibody is equivalent to Ser757 in mice.

实验方案

Product Specific Protocols
WB protocol for Phospho-ULK1 (Ser758) antibody 82776-8-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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