验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, A431 cells, HEK-293 cells, HepG2 cells |
| Positive IF/ICC detected in | MCF-7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
82730-10-RR targets IRS1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | IRS1 fusion protein Ag11714 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | insulin receptor substrate 1 |
| 别名 | HIRS 1, insulin receptor substrate 1, IRS 1, IRS-1 |
| 计算分子量 | 1242 aa, 132 kDa |
| 观测分子量 | 160-185 kDa |
| GenBank蛋白编号 | BC053895 |
| 基因名称 | IRS1 |
| Gene ID (NCBI) | 3667 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P35568 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Ins receptor substrate 1 (IRS1) was the first cloned and characterized member of the IRS family which are involved in ins receptor (IR) and ins-like growth factor I receptor (IGF-IR) signaling. IRS1 is phosphorylated by ins receptor tyrosine kinase and is involved in various cellular processes including DNA repair fidelity, transcriptional activity, and cell growth can support tumor development and progression. Mutations in this gene are associated with type II diabetes and susceptibility to ins resistance. IRS1 has a predicted molecular weight of 132 kDa, however, as a result of its extensive serine phosphorylation it separates on a SDS gel as a band of approximately 160-185 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for IRS1 antibody 82730-10-RR | Download protocol |
| IF protocol for IRS1 antibody 82730-10-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |